abstract: methylenetetrahydrofolate reductase (mthfr) is a key enzyme regulating folate metabolism, which affects dna methylation and synthesis. one of the most important polymorphisms identified in the mthfr gene is c677t. mthfr activity is lowered in individuals with 677tt genotype. using pyrosequencing, we analyzed the mthfr genotypes in 118 colorectal cancer patients and 189 normal matched ...

Interindividual differences in susceptibility to hematologic malignancies may be mediated in part through polymorphic variability in the bioactivation and detoxification of carcinogens. The glutathione S-transferases (GSTs) have been implicated as susceptibility genes in this context for a number of cancers. The aim of this study was to examine whether polymorphic variation in GSTs confers susc...

Molecular epidemiology and cross-sectional study. Multicenter from referral centers. 326 women were included. The primary outcome was designed of a kit for screening HPV-genotypes based on the prevalence found in population. Oligonucleotides different genotypes designed. conditions quantitative PCR pools low- high-risk (LR HR) HPVgenotypes established.

OBJECTIVE To detect the frequency and subtypes of HPV in the uterine cervix of HIV-infected women. METHODS Sample consisted of 288 HIV-infected women, recruited from the public health system of five cities of Minas Gerais, Brazil. Women were seen from August 2003 to August 2008. Cervical samples were collected for cytological analysis and for HPV DNA detection, using polymerase chain reaction...

The incidence of cervical cancer is expected to rise sharply in China. A reliable routine human papillomavirus (HPV) detection and genotyping test to be supplemented by the limited Papanicolaou cytology facilities is urgently needed to help identify the patients with cervical precancer for preventive interventions. To this end, we evaluated a nested polymerase chain reaction (PCR) protocol for ...

Esophageal cancer (EC) has a globally increasing incidence with poor curative treatment options and survival rates. Environmental and dietary factors have crucial roles in esophageal carcinogenesis. Polymorphisms in the UGT genes, a superfamily of enzymes essential for the detoxification of carcinogens, may alter enzyme activity and subsequently may play a role in EC etiology. Rather than solel...

Nitrosamine consumption and polymorphisms in CYP2E1, the product of which is involved in the activation of nitrosamines into reactive intermediates, have been shown to be associated with nasopharyngeal carcinoma (NPC) risk. Given that reactive intermediates created during nitrosamine metabolism are capable of DNA damage, we further hypothesized that differences between individuals in their abil...

A group of 208 human immunodeficiency virus (HIV)-infected women in Brazil were studied for the presence of human papillomavirus with the general SPF(10) PCR primer set. Virtually all (98%) women were found positive for human papillomavirus (HPV) DNA. Genotyping by the reverse hybridization line probe assay (HPV-LiPA) revealed a high prevalence of multiple genotypes (78.9% of the cases), with a...

INTRODUCTION The aim of this study was to analyze the distribution of human papillomavirus (HPV) genotypes in cytologically abnormal cervical samples from 106 women living in a region of the north of Spain. METHODS Cytological classification was reported according to the 2001 Bethesda System and HPV genotyping was performed by Roche Linear Array. RESULTS The overall HPV prevalence was 69.8%...