نتایج جستجو برای: high resolution melt analysis
تعداد نتایج: 4541240 فیلتر نتایج به سال:
We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.
During a survey of wild canids, internal transcribed spacer 1 real-time PCR and high-resolution melt analysis identified Leishmania tropica in samples from jackals and foxes. Infection was most prevalent in ear and spleen samples. Jackals and foxes may play a role in the spread of zoonotic L. tropica.
Current culture and phenotypic protocols for diagnosing Salmonella infections can be time-consuming. Here, we describe the application of a 16S rRNA PCR coupled to high-resolution melt analysis (HRMA) for species and serotype identification within 6 h of blood sample collection from a patient with Salmonella enterica serotype Enteritidis bacteremia.
We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species level, with 100% concordance to culture findings.
as the information carried in a high spatial resolution image is not represented by single pixels but by meaningful image objects, which include the association of multiple pixels and their mutual relations, the object based method has become one of the most commonly used strategies for the processing of high resolution imagery. this processing comprises two fundamental and critical steps towar...
Melt ponds, a common feature on Arctic sea ice, absorb most of the incoming solar radiation and have a large effect on the melting rate of sea ice, which significantly influences climate change. Therefore, it is very important to monitor melt ponds in order to better understand the sea ice-climate interaction. In this study, melt pond retrieval models were developed using the TerraSAR-X dual-po...
Introduction: Impact melt is a typical product produced during the cratering process as a result of the intense heating of the target usually because of pressure decompression, but also possible because of shear heating (Figure 1). Melt is observed on all of the terrestrial planets in both simple and complex craters [16]. High-resolution data for the Moon now allows impact melt deposits to be c...
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