Abstract A growing number of studies have investigated the viability feeding hemp by-products as livestock feedstuffs; however, their impact on microbiomes remains unexplored. Here, we evaluated effects hempseed cake (HSC) gastrointestinal, respiratory, and reproductive microbiota in beef heifers. Angus-crossbred heifers [19-months old; initial body weight (BW) = 494 ± 10 kg] were fed a corn-ba...