Nontreponemal antibody tests such as the Venereal Disease Research Laboratory (VDRL) test are carried out on serum and widely used as screening tests for syphilis. The aim of the present study was to develop a screening test for syphilis making use of whole blood and VDRL liposomes. Antibody to human red blood cells was conjugated to VDRL liposomes and reacted with a diluted sample of patient w...

Historically, surveillance for influenza A viruses (IAVs) in wild birds has relied on viral detection assays. This was largely due to poor performance of serological assays in wild birds; however, recently developed commercial serological assays have improved the ability to detect IAV antibodies in wild birds. Serological surveillance for IAV antibodies in Canada geese (Branta canadensis) has s...

We performed radioimmunoprecipitation assays in which iodinated preparations of A/Port Chalmers/73 (A/PC/73) hemagglutinin were used as the test antigens and high concentrations of unlabeled A/Hong Kong/68 viral protein were used to inhibit the binding of cross-reactive antibodies to quantitate strain-specific antibody responses in postvaccination sera. Strain-specific antibodies comprised 8 to...

The hemagglutination inhibition test is commonly used for the identification of newly isolated influenza viruses. In this laboratory several difficulties frequently were encountered in typing these new strains after primary isolation by means of this technique. These drawbacks were: (1) Some newly isolated strains are susceptible to nonspecific inhibitors of hemagglutination (Francis, Salk, and...

background and aims: influenza vaccination is one of the best way to prevent and control influenza worldwide. it is manufactured by who-licensed companies based on the who expertise committee annually. the aim of this study was partial quality control of the commercial human influenza vaccine 2008-9 and its matching with the circulating strains. materials and methods: the trivalent imported vac...

Five erythrocyte species (horse, goose, chicken, guinea pig, and human) were used to agglutinate avian influenza H5N1 viruses by hemagglutination assay and to detect specific antibody by hemagglutination inhibition test. We found that goose erythrocytes confer a greater advantage over other erythrocyte species in both assays.

The A/Los Angeles/2/87 (H3N2) (A/LA/2/87) virus is sensitive to inhibitors of hemagglutination present in certain human sera. It was found that the effect of these inhibitors could be removed by treating sera with high-concentration receptor-destroying enzyme or trypsin-periodate or by using inhibitor-resistant viruses in the hemagglutination inhibition (HAI) test. Inhibitor-resistant viruses w...

BACKGROUND Little is known about the development of cross-reactive antibodies following natural exposure to pathogens. Such knowledge is critical in the development of new universal influenza vaccines. METHODS To study the possibility of the presence of cross-reactive antibodies to influenza viruses which underwent a major antigenic drift between the years 1999 and 2007 sera from samples of 8...

Single radial diffusion (SRD) tests for antibodies to influenza type A hemagglutinin, neuraminidase, nucleoprotein, and matrix protein antigens were compared with conventional hemagglutination inhibition, neuraminidase inhibition, and complement fixation tests. Sera used in this study were obtained in 1968-1969 from volunteers before and after vaccination and before and after an ensuing epidemi...

In the spring and summer 2014, an outbreak of seal influenza A(H10N7) virus infection occurred among harbor seals (Phoca vitulina) off the coasts of Sweden and Denmark. This virus subsequently spread to harbor seals off the coasts of Germany and the Netherlands. While thousands of seals were reported dead in Sweden, Denmark and Germany, only a limited number of seals were found dead in the Neth...