Activation of phospholipase C (PLC)-mediated signaling pathways in non-excitable cells causes the release of calcium (Ca2+) from inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular Ca2+ stores and activation of Ca2+ influx via plasma membrane Ca2+ channels. The properties and molecular identity of plasma membrane Ca2+ influx channels in non-excitable cells is a focus of intense investi...

Inflammasomes are molecular complexes that initiate innate immune response. They are mainly expressed by immune cells; however, molecular manipulations in these cells remain very difficult. Here, we describe a simple protocol to overexpress and activate functional NRLP3 inflammasomes in HEK293 cells.

Abstract Biosynthesis of nanoparticles is the preferred route for fabrication biocompatible and cheaper drugs. In this study, extract major secondary metabolite from Helichrysum aureonitens , 3,5-dihydroxy-6,7,8-trimethoxyflavone, were used to synthesize silver zinc oxide nanoparticles. Spectroscopic microscopic techniques confirmed formation The flavone alone showed higher DPPH radical scaveng...

Lentiviral (LV) vectors have emerged as powerful tools for treating genetic and acquired human diseases. As clinical studies commercial demands progressed, there has been a growing need large amounts of purified LV vectors. To help meet this demand, we developed CRISPR library screening methods to identify perturbations in embryonic kidney 293 (HEK293) cells their derivatives that may increase ...

Objective: Biebersteinia (Geraniaceae) has a history of use in traditional medicine in some countries including Iran. In the present study, cytotoxic and apoptogenic properties of hydro-ethanol extract of B. multifidi was investigated on human prostate cancer cell lines (PC3 and DU 145) and human embryonic kidney 293 (HEK293) cells. Materials and Methods: Cells were cultured in RPMI-1640 medium...

RT-PCR confirmed that human cell lines of diverse peripheral origins express transcripts for the serotonin transporter (sert/slc6a4). Molecular weights reported for the translated protein appear to be quite variable however. Here we compared directly immunoreactive protein generated from cloned sert transfected into HEK293 with that carried endogenously among the cell lines. The dominant glycos...