1 Congregation for the Doctrine of the Faith, Donum vitae (February 2, 1987), Introduction, 5. 2 Ibid., I, 1. The topic of embryonic stem cells is very much in the forefront of public interest. The moral stance on obtaining stem cells from embryos is clear: The Church has always taught that all human life from the very beginning must be respected and protected. If human embryos are killed to ob...

Two of the common mechanisms regulating G protein-coupled receptor (GPCR) signal transduction are phosphorylation and sequestration (internalization). Agonist-mediated receptor phosphorylation by the beta-adrenergic receptor kinase (betaARK) facilitates subsequent interaction with an arrestin protein, resulting in receptor desensitization. Studies of the beta2-adrenergic receptor (beta2AR) rece...

The strategy of the nucleophilic substitution hydrogen (SNH) was first applied for metal-free C-H/C-H coupling reactions 4H-imidazole 3-oxides with indoles. As a result, series novel bifunctional azaheterocyclic derivatives were obtained in yields up to 95%. In silico experiments on molecular docking performed evaluate binding possibility synthesized small molecules selected biotargets (BACE1, ...

Gain/loss of function studies were utilized to assess the potential role of the endogenous vanilloid receptor TRPV4 as a sensor of flow and osmolality in M-1 collecting duct cells (CCD). TRPV4 mRNA and protein were detectable in M-1 cells and stably transfected HEK-293 cells, where the protein occurred as a glycosylated doublet on Western blots. Immunofluorescence imaging demonstrated expressio...

Overexpression of the ATP-binding cassette transporter ABCG2 reportedly causes multidrug resistance, whereas altered drug-resistance profiles and substrate specificity are implicated for certain variant forms of ABCG2. At least three variant forms of ABCG2 have been hitherto documented on the basis of their amino acid moieties (i.e., arginine, glycine and threonine) at position 482. In the pres...

Sialylation of N-glycans associated with recombinant human acetylcholinesterase (rHuAChE) has a central role in determining its circulatory clearance rate. Human embryonal kidney 293 (HEK-293) cells, which are widely used for the expression of recombinant proteins, seem to be limited in their ability to sialylate overexpressed rHuAChE. High-resolution N-glycan structural analysis, by gel permea...