Molecular hybridisation using cloned hepatitis B virus DNA (HBV DNA) was applied to liver and serum samples from 46 children (39 with liver diseases and seven controls) for detection of HBV DNA sequences, free and integrated into the liver cell genome. HBV DNA integration was observed in 10 children. The young age of some of these cases indicates that such integration can occur early in liver d...

introduction: the hepatitis b is a viral infection that causes a big problem globally. about 2 billion people worldwide are infected and there are now about 400 million hbv-dna carriers around the world. hbv infection is the ninth cause of death worldwide and infects about 350 million new cases each year in the world. hbv-dna can be spotted in different body secretions and fluids, including ser...

Background: HBV and HCV coinfection is common, particularly in endemic areas and among high risk groups. In this study we have investigated the prevalence of HBV/HCV coinfection and compared the biochemical and serological characteristics of such patients compare with the patients having hepatitis C infection alone. Methods: We studied 207 patients diagnosed as having chronic hepatitis C, with ...

The recognition of replicating hepatitis B virus (HBV) may be important to both define the cause of and know how to manage chronic liver disease in multitransfused hemophilic patients. Replicating HBV can be detected at the molecular level by methods for HBV-specific DNA (HBV-DNA), which are much more sensitive than the immunologic methods for detecting hepatitis B surface antigen (HBsAg) and h...

Quantitation of hepatitis B virus (HBV) DNA in serum is a useful method for the monitoring of HBV replication. We attempted to develop a quantitative assay system for HBV DNA that is more sensitive, accurate, and reproducible than existing systems. We detected HBV DNA by real-time detection PCR (RTD-PCR) based on Taq Man chemistry. The efficacy of this assay was evaluated by quantitatively meas...

background although certain hbv mutations are known to affect the expression of hepatitis e antigen, their association with hbv viral level or clinical outcomes is less clear. objectives we evaluated associations between different mutations in the basal core promoter (bcp) and pre-core (pc) regions of hbv genome and subsequent changes in hbv viral dna level over seven years in a population of u...

Nucleos(t)ide analogues (NAs) lead to viral suppression and undetectable hepatitis B virus (HBV) DNA in some individuals infected with HBV, but the rate of virological rebound has been unknown in such patients. We examined the prevalence of virological rebound of HBV DNA among NA-treated patients with undetectable HBV DNA. We retrospectively analyzed 303 consecutive patients [158 entecavir (ETV...

AIM To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen. METHODS Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QIAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based re...

During previous studies of susceptibility to hepatitis B virus (HBV) infection, HBV DNA was detected in 2/6 wild-caught baboons. In the present study, HBV DNA was amplified from 15/69 wild-caught baboons. All animals were negative for HBV surface antigen and antibody against HBV core antigen. Liver tissue from 1 baboon was immunohistochemically negative for HBV surface antigen but positive for ...