We report a novel method for the non-destructive handling of, and biochemical experiments with, individual intact chromatin fibers, as well as their isolation from single cells, utilizing a specifically designed microfluidic device with an optically driven microtool under the microscope. Spheroplasts of recombinant fission yeast cells expressing fluorescent protein-tagged core histones were emp...

The rotary valve is a widely used mechanical device in many solids-handling industrial processes. However, it may also be responsible for most of the attrition effects occurring in a typical process. In this study, the attrition effects occurring in a rotary valve operating as a stand alone device and as part of a pneumatic conveying system are investigated. The Gwyn’s correlation was found to ...

A general device for handling nondeterminism in stack operations is described. The device, called a Graph-structured Stack, can eliminate duplication of operations throughout the nondeterministic processes. This paper then applies the graph-structured stack to various natural language parsing methods, including ATN, LR parsing, categodal grammar and principlebased parsing. The relationship betw...

The design and use of a new device for obtaining corneoscleral buttons for storage and corneal discs for keratoplasty from neonatal and adult donor eyes is described. The device is simple to use and eliminates most of the difficulties in handling infant donor eyes. It thus minimises the risk of damage to the corneal endothelium and the formation of stretch striae, which reduce the quality of th...

We describe a new cable-free, non-telemetric method for synchronized electrophysiological and video recordings of seizure activity in freely moving mice. The electrophysiological recordings were made by a head-mounted 4-channel data-logging device, allowing the mouse to move freely in its cage, and even to be moved from cage to cage under ongoing recording. Seizures were studied in Synapsin I/I...

Droplet microfluidics (DMF) is a fluidic handling technology that enables precision control over dispensing and subsequent manipulation of droplets in the volume range of microliters to picoliters, on a micro-fabricated device. There are several different droplet actuation methods, all of which can generate external stimuli, to either actively or passively control the shape and positioning of f...

BACKGROUND In patients receiving inhaled medication, dissatisfaction with and difficulty in using the inhaler can affect treatment adherence. The incidence of handling errors is typically higher in the elderly than in younger people. The aim of the study was to assess inhaler preference for and handling errors with the ELLIPTA® dry powder inhaler (DPI), (GSK), compared with the established BREE...