The plasma membrane H-ATPase generates an electrochemical gradient of H across the plasma membrane that provides the driving force for solute transport and regulates pH homeostasis and membrane potential in plant cells. Recent studies have demonstrated that phosphorylation of the penultimate threonine in H-ATPase and subsequent binding of a 14-3-3 protein is the major common activation mechanis...

Introduction Phosphorus (P) deficiency hinders cotton ( Gossypium hirustum L.) growth and development, seriously affecting lint yield fiber quality. However, it is still unclear how P fertilizer affects length. Methods Therefore, a two-year (2019-2020) pool-culture experiment was conducted using the split-plot design, with two cultivars (CCRI-79; low-P tolerant SCRC-28; sensitive) as main plot....

N-ethymaleimide-sensitive ATPase activity was measured in crude homogenates of gill tissue from rainbow trout using a coupled-enzyme ATPase assay in the presence of EGTA, ouabain and azide. This NEM-sensitive ATPase activity, determined to be about 1.5 mmolmg21 protein h21 at 15 ̊C for freshwater trout, is also inhibited by other H+ATPase blockers such as DCCD, DES, PCMBS and bafilomycin. It is ...

We previously have demonstrated that the colonic P-ATPase alpha subunit cDNA encodes an H,K-ATPase when expressed in Xenopus laevis oocytes. Besides its high level of amino acid homology (75%) with the Na,K-ATPase, the colonic H,K-ATPase also shares a common pharmacological profile with Na,K-ATPase, because both are ouabain-sensitive and Sch 28080-insensitive. These features raise the possibili...