We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein.

BACKGROUND: Lactobacilli are known as a valuable sourceof antimicrobial compounds and have a high potential of use infood biopreservation against food related microorganisms.OBJECTIVES: Antimicrobial potency of 63 dairy lactobacilliisolates against four highly important food-related microorganismswere evaluated. In addition, a new way in data organization wasintroduced, which led to a more info...

In the educational context it is examined the teacher-stress association. The teacher’s stress is related with variables of academic environment, of out of the school and of the teacher. The impact of that association can be see in the teacher’s health, in his achievement and in the quality of the teaching-learning process. With the objective to present one view of how issue is being considered...

We demonstrate that an oft-used indirect attitude assessment technique—the attitude activation paradigm—accurately assesses attitudes only when participants attend to the prime stimuli during the attitude activation task. Attitude activation attitudes toward obviously valenced words (e.g., torture, liberty) were more sensitive to attitude valence and extremity when participants were required to...

An adaptive method is employed to speed up computation of high accuracy surface modeling (HASM), for which an error indicator and an error estimator are developed. Root mean-square error (RMSE) is used as the error estimator that is formulated as a function of gully density and grid cell size. The error indicator is developed on the basis of error surfaces for different spatial resolutions, whi...

This paper presents some aspects related to the issues of aggregating economic indicators. Departing from the research of Gh. Paun (1983) we will prove a theorem which states that under certain, natural assumptions, it is impossible to obtain an optimum aggregation. Unlike the original work of Paun, in this paper we are giving a rather simple proof of this theorem.

The validity of the Michaelis-Menten-Briggs-Haldane approximation for single enzyme reactions has recently been improved by the formalism of the total quasi-steady-state approximation. This approach is here extended to fully competitive systems, and a criterion for its validity is provided. We show that it extends the Michaelis-Menten-Briggs-Haldane approximation for such systems for a wide ran...

In December 2003, seventeen years after the first UK research assessment exercise, Italy started up its first-ever national research evaluation, with the aim to evaluate, using the peer review method, the excellence of the national research production. The evaluation involved 20 disciplinary areas, 102 research structures, 18,500 research products and 6,661 peer reviewers (1,465 from abroad); i...

Ameisen: Die heimliche Weltmacht (Ants: Nature's Secret Power) is a nature documentary about ants. Wolfgang Thaler wrote, filmed, and directed the film, which focuses on the work of ant researcher Bert Hölldobler. The 2004 film was produced by Adi Mayer Film for Österreichischer Rundfunk (Austrian Broadcasting), a public service broadcaster headquartered in Vienna, Austria. Ants: Nature's Secre...

background when a primary radiation interacts with a medium, the secondary radiations are produced, that the contribution of these secondary radiation should considered in the calculation of dose. build-up factor is a dosimetric correction factor to assume effect of secondary radiation in calculation of dose. calculation of this factor is very difficult and time consuming. due to significance o...