ghrelin is a peptide hormone that is composed of 26 amino acids and leads to a positive energy balance and obesity through stimulation of releasing growth hormone, and increasing food intake and body weight.  the purpose of this study was to determine and evaluate the association between the ghrelin gene polymorphism and growth traits in ross and cobb broiler chickens. blood samples were collec...

camel is a resistant species to drought and cold weather and produces milk and meat in harshest environmental condition. camel population in iran has been decreasing during last decades, and genetic studies are quite necessary for considering the conservation and productivity of this species. in this study growth hormone gene influencing growth, milk production and reproduction was considered. ...

Using restriction endonuclease analysis and a human growth hormone cDNA probe, we have found a Chinese family with a human growth hormone gene deletion. Two affected sibs are homozygous for a deletion of approximately 7.1 kb of DNA, which contains the normal human growth hormone gene. The patients' parents and grandmothers are heterozygous for the deleted gene. Their grandfathers are normal and...

A region of the rat growth hormone gene and 5' flanking DNA has been identified which promotes accurate, thyroid hormone-regulated transcriptional initiation. GC rat pituitary tumor cells were transfected with chimaeric plasmids containing various lengths of rat growth hormone gene and 5' flanking DNA fused to the coding region of the dominant selectable marker gene neo. Thyroid hormone inducti...

conclusions while amh receptor expression was higher in experimental rats, their serum concentrations of amh were decreased. further researches with greater sample sizes and measurement of bioactive forms of hormones are recommended to confirm the findings of this study. background anti-mullerian hormone (amh) levels reflect the number of small antral follicles in ovaries and expression changes...

Stably transfected cell lines containing the normal human growth hormone (hGH-N) and human growth hormone-variant (hGH-V) genes have been established in order to study the expression of these two highly homologous genes. Each gene was inserted into a bovine papillomavirus shuttle vector under the transcriptional control of the mouse metallothionein gene promoter and the resultant recombinants w...

Human growth hormone (hGH) genomic sequence containing 5 exons and 4 introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH in stably transfected cells was assayed using ELISA. Total RNA was extracted from transfected cells and hGH cDNA was amplified by RT-PCR using specific primers...

Background Follicle stimulating hormone (FSH)- a hetrodimeric glycoprotein- is secreted by pituitary gland. This hormone stimulates growth and maturation of the follicles in females and sperms in male. Up to now, glycoprotein hormones such as FSH have produced in different cell lines. Among of the mammalian expression systems, the Chinese hamster ovary cells (CHO) have taken into consideration ...