The febrile response to bacterial endotoxin was measured in rabbits made leukopenic with nitrogen mustard. A striking increase in susceptibility to the lethal effects of endotoxin occurred in severely leukopenic animals. Animals without circulating granulocytes, or with only basophils, developed no significant fever after endotoxin injection. Animals with circulating granulocytes other than bas...

The present studies were carried out to (1) evaluate a leukoadhesive technique for obtaining granulocytes for transfusion, (2) assess the granulocytes by in vitro techniques, and (3) determine the efficacy of granulocyte transfusion in preventing sepsis in leukopenic dogs. Dogs were rendered transiently leukopenic (<500 per cu mm) by intravenous cyclophosphamide, 40 mg/kg. Quantitative and qual...

The treatment of healthy donors with granulocyte colony-stimulating factor (G-CSF) and dexamethasone results in sufficient numbers of circulating granulocytes to prepare granulocyte concentrates for clinical purposes. Granulocytes obtained in this way demonstrate relatively normal functional behavior combined with a prolonged life span. To study the influence of mobilizing agents on granulocyte...

The functional properties of granulocytes in a diabetic patient deficient in myeloperoxidase (MPO) were compared with those of granulocytes in healthy subjects. The granulocytes of this patient had normal phagocytic activity. The microbicidal activity of the granulocytes was partially diminished with regard to Staphylococcus aureus and was almost nil with regard to Candida albicans. Fungicidal ...

Phagocytosis and intracellular killing of Staphylococcus aureus by granulocytes were examined in diabetic patients. There was no significant difference in the phagocytic activity of granulocytes between control and diabetic-subjects. However, intracellular killing by granulocytes was significantly reduced in insulin-treated diabetic patients compared with control subjects. No significant differ...

BACKGROUND The present study was designed to investigate the hypothesis that cells from ill patients and from healthy subjects may have different reactivities under metabolic stimulation. METHODS The study was performed with granulocytes from non-diabetic subjects and from type II -Non Insulin Dependent Diabetes mellitus (NIDDM) patients. The nitric oxide (NO) generation was comparatively det...

Phagocytosis of quartz particles by rabbit alveolar macrophages and monocytes and human granulocytes and monocytes was accompanied by stimulation of substrate free reduction of nitroblue tetrazolium to formazan. This reflects activation of an oxygen dependent bactericidal system of phagocytes and total (exogenic and endogenic) generation of active oxygen species. Low fibrogenic and cytotoxic al...

The behaviour of peripheral blood granulocyte pools, nitroblue tetrazolium reduction by granulocytes, migration of granulocytes out of skin windows, serum muramidase activity and bone marrow reserve of granulocytes during uncomplicated bronchopneumonia were studied. The time course of changes in these parameters during infection was established, and suggests maximal changes on day 2 of infectio...

The functional capacities of granulocytes in patients with chronic granulocytic leukemia are still a subject of controversy, probably due to the heterogeneity of the abnormalities observed from patient to patient. For a better definition of these abnormalities, 14 patients with untreated chronic granulocytic leukemia were studied. The patients were divided into three groups on the basis of the ...

The effect of different leukocyte antibodies on the fate in vivo of granulocytes is not known. Thus, the optimum in vitro serologic tests to determine a safe and effective granulocyte transfusion or to diagnose immune destruction of granulocytes in other clinical situations have not been identified. We have studied the effect of granulocyte agglutinating (GA), granulocytotoxic (GC), and lymphoc...