The Drosophila sugarless and sulfateless genes encode enzymes required for the biosynthesis of heparan sulfate glycosaminoglycans. Biochemical studies have shown that heparan sulfate glycosaminoglycans are involved in signaling by fibroblast growth factor receptors, but evidence for such a requirement in an intact organism has not been available. We now demonstrate that sugarless and sulfateles...

1. The effect of MgCl(2) concentration on the interaction of Alcian Blue 8GX and glycosaminoglycans in the urine of patients with mucopolysaccharidosis was studied by using a new quantitative micro method for the measurement of Alcian Blue-glycosaminoglycan complexes. This provided a means of measuring the critical electrolyte concentrations of urinary glycosaminoglycans. 2. Theoretical conside...

Qualitative and quantitative determinations of glycosaminoglycans in the nonswelling corneal stroma of the spiny dogfish shark, Squalus acanthias, were made with the use of a cetylpyridinium chloride-cellulose microfractionation technique. Dogfish stroma was found to contain predominantly highly sulfated galactosaminoglycans forming approximately 75 per cent of the total glycosaminoglycans when...

The relative binding affinity of Zn2+ to several glycosaminoglycans was determined by gel-filtration chromatography. Binding was observed only between Zn2+ and heparin. No binding was observed between Zn2+ and chondroitin 4-sulphate, chondroitin 6-sulphate, dermatan sulphate or hyaluronic acid. All of the glycosaminoglycans contained carboxy groups, but only heparin bound Zn2+. This observation...

A large amount of glycosaminoglycans was found in the AH-130 ascites tumor cells and also in the ascites fluids. After combined administration of Cyclophosphamide and vitamin A to the tumor-bearing rats, a significant decrease was found in tumor glycosaminoglycans sensitive to lysosomal hyaluronidase, such as the nonsulfated glycosamino glycans or chondroitin sulfate A and/or C. An increased re...