OBJECTIVE To evaluate the involvement of proinflammatory and oxidative stress markers in gingival tissue in individuals with chronic periodontitis. SUBJECT AND METHODS Eighteen subjects were divided in two groups: experimental (age 52.9+/-5.0) and control (age 51.1+/-9.6). The activities of enzymatic antioxidants such as catalase, glutathione peroxidase (GPx), glutathione S-transferase (GST),...

This article presents a reliable, effective and easy procedure for measuring glutathione peroxidase (Gpx) activity. Enzyme samples were incubated with phosphate buffer, which included the appropriate concentrations of (GSH) peroxide as substrates, to determine Gpx In CUPRAC method, reagent Cu(Nc)22+ was added stop enzymatic reaction after sufficient period incubation. The unreacted substrates a...

Numerous studies indicate a role for oxidative stress in the neuronal degeneration and cell death that occur during ischemia-reperfusion injury. Recent data suggest that inhibition of the proteasome may be a means by which oxidative stress mediates neuronal cell death. In the current study, the authors demonstrate that there is a time-dependent decrease in proteasome activity, which is not asso...

CAT: Catalase; SOD: Superoxide Dismutase; GPx: Glutathione Peroxidase; GST: Glutathione-S-Transferase; GSH: Glutathione; LPO: Lipid Peroxidation; MDA: Malondialdehyde; H2O2: Hydrogen Peroxide; D.H2O: Distilled Water; Conc.: Concentrated; ROS: Reactive Oxygen Sspecies; RBC: Red Blood Cells ; NADH: Nicotinamide Adenine Dinucleotide;CDNB: 1-Chloro-2,4-Dinitro Benzene; TCA: Trichloro Acetic Acid; E...

We showed previously that the selenium-dependent glutathione peroxidase, GPX-GI, encoded by the Gpx2 gene, is highly expressed in the epithelium of the gastrointestinal (GI) tract and sporadically in breast tissue. To investigate whether Gpx2 gene expression is epithelium specific, we used in situ hybridization to show that Gpx2 mRNA is highly expressed in the crypt epithelium of human intestin...

The present study aimed to evaluate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rats. Forty male rats were randomly divided into sham, control (I/R injury) and three crocin-pretreated groups. To induce I/R lesions, the celiac artery was clamped for 30 min and then the clamp was removed to allow reperfusion for 3 h. Pretreated-rats re...

Numerous experimental and clinical analyses of biomechanical injury have served to elucidate the pathophysiologic events involved in Traumatic Brain Injury(TBI).Secondary insults activate the release of cellular mediators including proinflammatory cytokines,prostaglandins, free radicals,and complement.The resulting cascade of events avalanche into free radical induced oxidative damage to the br...

Summary The study aimed to investigate glutathione peroxidase (GPx) activity and (GSH) levels in the sperm of patients with unexplained infertility. samples were collected from subjects normal semen parameters divided into fertile infertile groups. Sperm analysis was performed according 2010 WHO criteria. Measurement GPx GSH by enzymatic assay kits. higher recorded spermatozoa seminal plasma gr...

Erectile dysfunction (ED) drugs have been used for treatment of erectile dysfunction which affects the lives of approximately 300 million men worldwide. It is well known that the cytosolic glutathione Stransferase (GST) and glutathione metabolizing enzymes play an important role in the detoxification of many endogenous and exogenous compounds. Therefore, the present study aims at investigating ...

Submit Manuscript | http://medcraveonline.com J Aquac Mar Biol 2015, 2(4): 00035 Abbreviations: SOD: Superoxide Dismutase; CAT: Catalase; TAC: Total Antioxidant Capacity; MDA: Malondialdehyde; ROO: Primarily Peroxyl Radicals; GPX: Glutathione Peroxidase; ROS: Reactive Oxygen Species; GST: Glutathione-S-Transferase; GR: Glutathione Reductase; PPT: Parts Per Trillion; Ppm: Parts Per Million; RPM:...