نتایج جستجو برای: glutathione disulfide gssg

تعداد نتایج: 57687  

Journal: :The international journal of biochemistry & cell biology 1995
O Benard K A Balasubramanian

Oxidative stress is associated with the formation of oxidized glutathione (GSSG) in the cells, which can form mixed disulfide with proteins leading to alteration of their function. The present study looks at the effect of in vitro exposure of GSSG on intestinal mitochondria and brush border membrane (BBM). Incubation with 1 mM GSSG increased the protein bound GSH in mitochondria by 15-fold. Thi...

Journal: :Clinical chemistry 2002
Ranieri Rossi Aldo Milzani Isabella Dalle-Donne Daniela Giustarini Lorenzo Lusini Roberto Colombo Paolo Di Simplicio

BACKGROUND The reported mean concentration of glutathione disulfide (GSSG) in human blood/erythrocytes varies widely (1 to >500 micromol/L), as does that of reduced glutathione (GSH) to a lesser extent. We have identified and investigated possible pitfalls in measurement of both GSH and GSSG. METHODS We measured GSH and GSSG using a spectrophotometer with a modification of the GSH recycling m...

Journal: :Proteomics 2007
Toru Ezure Takashi Suzuki Masamitsu Shikata Masaaki Ito Eiji Ando Osamu Nishimura Susumu Tsunasawa

Escherichia coli alkaline phosphatase (AP) and human lysozyme (h-LYZ), which contain two and four disulfide bonds, respectively, were expressed in a cell-free protein synthesis system constructed from Spodoptera frugiperda 21 (Sf21) cells. AP was expressed in a soluble and active form using the insect cell-free system under non-reducing conditions, and h-LYZ was expressed in a soluble and activ...

2013
Ruchaneekorn W. Kalpravidh Thongchai Tangjaidee Suneerat Hatairaktham Ratiya Charoensakdi Narumol Panichkul Noppadol Siritanaratkul Suthat Fucharoen

β -thalassemia/Hb E is known to cause oxidative stress induced by iron overload. The glutathione system is the major endogenous antioxidant that protects animal cells from oxidative damage. This study aimed to determine the effect of disease state and splenectomy on redox status expressed by whole blood glutathione (GSH)/glutathione disulfide (GSSG) and also to evaluate glutathione-related resp...

2013
Joseph Brock Philip G. Board Aaron J. Oakley

Glutathione transferases (GSTs) are dimeric enzymes containing one active-site per monomer. The omega-class GSTs (hGSTO1-1 and hGSTO2-2 in humans) are homodimeric and carry out a range of reactions including the glutathione-dependant reduction of a range of compounds and the reduction of S-(phenacyl)glutathiones to acetophenones. Both types of reaction result in the formation of a mixed-disulfi...

Journal: :Chemical research in toxicology 2004
Liuji Chen XianQiang Yang Hongli Jiao Baolu Zhao

Our previous research showed that tea catechins could significantly increase the viability of lead-exposed PC12 cells. Whereas the cellular thiol status is known to be responsible for protecting against lead-induced toxicity, whether the role of tea catechins on lead-induced PC12 cell toxicity is related to the metabolism of intracellular thiol compounds remained vague. In the present study, it...

Journal: :Applied and environmental microbiology 1997
M T Elskens M J Penninckx

A rapid decrease of intracellular glutathione (GSH) was observed when exponentially growing cells of Saccharomyces cerevisiae were treated with sublethal concentrations of either dimethyldithiocarbamic acid or thiram [bis(dimethylthiocarbamoyl) disulfide]. The underlying mechanism of this effect possibly involves the intracellular oxidation of dimethyldithiocarbamate anions to thiram, which in ...

Journal: :FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009
S Jill James Shannon Rose Stepan Melnyk Stefanie Jernigan Sarah Blossom Oleksandra Pavliv David W Gaylor

Research into the metabolic phenotype of autism has been relatively unexplored despite the fact that metabolic abnormalities have been implicated in the pathophysiology of several other neurobehavioral disorders. Plasma biomarkers of oxidative stress have been reported in autistic children; however, intracellular redox status has not yet been evaluated. Lymphoblastoid cells (LCLs) derived from ...

Journal: :American journal of physiology. Regulatory, integrative and comparative physiology 2007
Junqiang Tian Naohiro Washizawa Li H Gu Marc S Levin Lihua Wang Deborah C Rubin Simon Mwangi Shanthi Srinivasan Yuhao Gao Dean P Jones Thomas R Ziegler

Limited data in animal models suggest that colonic mucosa undergoes adaptive growth following massive small bowel resection (SBR). In vitro data suggest that intestinal cell growth is regulated by reactive oxygen species and redox couples [e.g., glutathione (GSH)/glutathione disulfide (GSSG) and cysteine (Cys)/cystine (CySS) redox]. We investigated the effects of SBR and alterations in redox on...

Journal: :American journal of physiology. Lung cellular and molecular physiology 2009
Smita S Iyer Allan M Ramirez Jeffrey D Ritzenthaler Edilson Torres-Gonzalez Susanne Roser-Page Ana L Mora Kenneth L Brigham Dean P Jones Jesse Roman Mauricio Rojas

Several lines of evidence indicate that depletion of glutathione (GSH), a critical thiol antioxidant, is associated with the pathogenesis of idiopathic pulmonary fibrosis (IPF). However, GSH synthesis depends on the amino acid cysteine (Cys), and relatively little is known about the regulation of Cys in fibrosis. Cys and its disulfide, cystine (CySS), constitute the most abundant low-molecular ...

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