CYP83A1 and CYP83B1 are two key synthesis genes in the glucosinolate biosynthesis pathway. CYP83A1 mainly metabolizes the aliphatic oximes to form aliphatic glucosinolate and CYP83B1 mostly catalyzes aromatic oximes to synthesis corresponding substrates for aromatic and indolic glucosinolates. In this study, two CYP83A1 genes named BcCYP83A1-1 (JQ289997), BcCYP83A1-2 (JQ289996) respectively and...

Seven groups of 10 growing rats each were fed a control diet or rapeseed diets with glucosinolate contents ranging from 4.4 to 36.6 mM/kg DM. After a 5-day adaptation period, the rats were fed ad libitum for 17 days. The food intake of the experimental groups was maximal after 11 days and was, on an average, 4 to 45% lower than that of the control group. The differences were still greater durin...

One of the best-studied plant defense systems, the glucosinolate-myrosinase system of the Brassicales, is composed of thioglucosides known as glucosinolates and their hydrolytic enzymes, the myrosinases. Tissue disruption brings these components together, and bioactive products are formed as a consequence of myrosinase-catalyzed glucosinolate hydrolysis. Among these products, isothiocyanates ha...

Glucosinolates are natural plant products that function in the defense toward herbivores and pathogens. Plant defense is regulated by multiple signal transduction pathways in which salicylic acid (SA), jasmonic acid, and ethylene function as signaling molecules. Glucosinolate content was analyzed in Arabidopsis wild-type plants in response to single or combinatorial treatments with methyljasmon...

Intraspecific variation in plants plays a major role in the composition and diversity of the associated insect community. Resistance traits of plants are likely candidates mediating community composition. However, it is debated whether total concentrations of chemical compounds or specific compounds determine herbivore resistance, and how chemical diversity among plant genotypes in turn affects...

Glucosinolates have anti-carcinogenic properties. In the recent decades, the genetics of glucosinolate biosynthesis has been widely studied, however, the expression of specific genes involved in glucosinolate biosynthesis under exogenous phytohormone treatment has not been explored at the subspecies level in Brassica oleracea. Such data are vital for strategies aimed at selective exploitation o...

Glucosinolates are Brassicaceae-specific secondary metabolites that act as crop protectants, flavor precursors, and cancer-prevention agents, which shows strong evidences of anticarcinogentic, antioxidant, and antimicrobial activities. MYB28, the R2R3-MYB28 transcription factor, directly activates genes involved in aliphatic glucosinolate biosynthesis. In this study, the MYB28 homology (BoaMYB2...

Models predicting optimal levels of plant defense against herbivores typically include two assumptions: 1) defense is both beneficial and costly; and 2) the relationship between costs and benefits of a defense is consistent across environments. However, the expression of costs and benefits of defense may be environmentally dependent. We examined lines of Brassica rapa, previously divergently se...

Broccoli (Brassica oleracea L. Italica Group) is an economically important vegetable crop and its consumption may benefit human health. Glucosinolates, a group of secondary plant metabolites found generally in the cultivated Brassicaceae, may protect against the development of certain malignancies. The objective of this study was to evaluate total and individual glucosinolate content of broccol...

It is widely accepted that the distinctive aroma and flavour traits of Brassicaceae crops are produced by glucosinolate (GSL) hydrolysis products (GHPs) with other non-GSL derived compounds also reported to contribute significantly their aromas. This study investigated profile content four species (salad rocket, horseradish, wasabi, watercress). Solid-phase microextraction followed gas chromato...