Urinary N-acetyl-β-D-glucosaminidase is a marker of early tubular damage. Therefore, the current study was to investigate effects and its underling mechanisms valsartan on gentamicin induced renal injury through urinary parameter variety in rats. Animals were divided into four groups consisting 12 rats each. The lasted for 10 d. Rats treated two batches 6th 11th d experiment, with 6 each group....

Human jejunal biopsy slices were maintained in culture for up to 48 hours. At 24 hours there was good morphological preservation but by 48 hours there was ultrastructural evidence of damage to the enterocytes. During culture the tissue had lost a certain amount of protein. At the same time the levels of three brush border enzymes (alkaline phosphatase, alpha-glucosidase, and leucyl-beta-naphthy...

1. Biopsies of rectal mucosa were obtained for histology and enzyme analysis from 32 patients with inflammatory and functional bowel disorders, and the biopsies were classified morphologically as active colitis, quiescent colitis or normal. 2. Supernatant fractions of biopsy homogenates were assayed for their content of the proteolytic enzymes alpha-chymotrypsin, elastase and cathepsin D, and o...

A purified monoclonal antibody (MAb) which specifically reacts with Staphylococcus aureus glucosaminidase was obtained. This MAb was utilized to develop an immunoenzymatic assay for the identification of S. aureus strains. The sensitivity of this assay, based on the simultaneous detection of S. aureus glucosaminidase and protein A, was evaluated by analyzing a total of 196 strains, 26 of which ...

1. Iron, acid phosphatase and N-acetyl-beta-glucosaminidase were assayed in liver biopsies from control subjects and patients with primary and secondary haemochromatosis. 2. The activities of the lysosomal enzymes were significantly higher in liver biopsies from patients with iron overload than in those from other patient groups. 3. Lysosomes from the livers of patients with iron overload were ...

Glycoproteins having mannose and/or N-acetylglucosamine in the terminal non-reducing position [Stockert, Morell & Scheinberg (1976) Biochem. Biophys. Res. Commun. 68, 988-993], and various lysosomal enzymes [Stahl, Schlesinger, Rodman & Doebber (1976) Nature (London) 264, 86-88] are rapidly cleared from plasma by the liver after intravenous administration. A liver cell-separation technique was ...

1. A collagenase digest of rat kidney cortex was separated into four bands by zonal centrifugation. 2. Two of these bands were shown by light-microscopy to contain glomeruli and tubular fragments, which were free from each other and well separated from other renal material. 3. Protein, N-acetyl-beta-glucosaminidase, 5'-nucleotidase, l-leucine beta-naphthylamidase, leucine aminopeptidase, acid p...

abstract   background: hydronephrosis is the most common congenital condition that is detected by prenatal ultrasonography. moreover, the widespread use of prenatal ultrasonography results in an increased recognition of fetal hydronephrosis. prenatal hydronephrosis is diagnosed at an incidence of 1:100 to 1:500 by ultrasonographic studies. the presence of hydronephrosis is not synonymous with o...