Hemoglobin is the iron-containing oxygen-transport metalloproteins in the red cells of the blood in mammals and other animals. A spheroidal heme protein having four subunits each consisting of a globular protein non-covalently bound, with an embedded heme group. Hb has a molecular weight of about 64456. The globular protein units of Hb is made up of two identical pairs of polypeptide chains, i....

background treatment with peginterferon alpha-2b plus ribavirin is the current standard therapy for chronic hepatitis c (chc). however, many host related and viral parameters are associated with different outcomes of combination therapy. objectives the aim of this study was to develop an artificial neural network (ann) model to predetermine individual responses to therapy based on patient’s dem...

Currently, invasive methods are used to measure the hemoglobin concentration and the most hemoglobin-derivatives, whereby blood is taken from the patient and subsequently analyzed. The noninvasive method presented here allows pain free continuous on-line patient monitoring with minimum risk of infection and facilitates real time data monitoring allowing immediate clinical reaction to the measur...

The reaction of hemoglobin with oxygen has been studied by stopped flow methods, and, under suitably restricted conditions, it can be adequately represented by a system of four consecutive reversible reactions. The numerical values given to the eight rate constants permit a satisfactory fit to combination and dissociation velocity data, and yield an equilibrium curve of the appropriate form. Th...

The reaction of hemoglobin with oxygen has been studied by stopped flow methods, and, under suitably restricted conditions, it can be adequately represented by a system of four consecutive reversible reactions. The numerical values given to the eight rate constants permit a satisfactory fit to combination and dissociation velocity data, and yield an equilibrium curve of the appropriate form. Th...

Using a millimolar absorptivity of 7.12 +/- 0.09 at 523 nm, it is possible to estimate the total concentration of hemoglobin in solutions containing oxyhemoglobin, deoxyhemoglobin and methemoglobin in any combination. This estimate is independent of the pH in the range 6.0-10.0 and will provide concentrations comparable to that obtained by the conventional and more precise use of cyanomethemogl...

1. When formaldehyde (0.10 M) is added to solutions of human hemoglobin, the oxygen affinity of the hemoglobin increases considerably (more than tenfold near pH 7). The interaction between hemes of the same hemoglobin molecule decreases, as shown by a drop in the value of n in Hill's equation from 2.9 to 1.5 or less. 2. In the presence of formaldehyde, both n and the oxygen pressure for half-sa...

Hemoglobin I was isolated from nodules formed on the roots of Parasponia andersonii inoculated with Rhizobium strain CP 283. The rate of oxygen dissociation from Parasponia hemoglobin increases about 12-fold between pH 4 and 7, with apparent pK 6.4, to reach a limiting value of 14.8s-1. The optical spectrum of oxyhemoglobin in the visible region is also dependent on pH with pK near 6.4. The rat...