The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.

In recent years it has become increasingly difficult to purchase ingredients for the preparation of various modifications of Romanowsky stains, such as Giemsa, Leishman and Wright's, at a reasonable cost; if indeed they can be obtained at all. During the past year, work has been in progress in the laboratories of the Malaria Institute of India with the object of producing a stain in which readi...

BACKGROUND Trichomonas vaginalis is a parasitic protozoan which causes most common non viral sexually transmitted disease trichomoniasis. Direct microscopic examination of vaginal fluid remains the most widely used diagnostic test. Although, wet mount examination is the most cost-effective diagnostic test, but it has low sensitivity resulting in under diagnosis of the disease. Therefore, to ove...

Aim—To determine whether two recently described staining methods (the modified McMullen’s and the Helicobacter pylori silver stain HpSS methods) used for the histological identification of H pylori organisms are superior to two established techniques (the modified Giemsa and anti-H pylori antibody immunostain) in terms of availability, reproducibility, rapidity, sensitivity, and cost. Methods—H...

Aim: Helicobacter pylori infection has been ascertained to play pivotal role in the pathogenesis of chronic gastritis and gastric neoplasia.1The present study was performed evaluate diagnostic accuracy H&E stain Giemsa for histological diagnosis helicobacter by taking immunohistochemical staining as a gold standard. Methods: A total 155 cases were included our study. The received biopsies f...

Fish eosinophilic granular cells are found in the connective tissues of structures associated with external environment. These similar to mammalian mast terms structural and functional characteristics. The cytoplasmic granules these give different staining reactions depending on fixative type. This study aimed determine properties densities Oscar fish (Astronotus ocellatus Agassiz, 1831) intest...

BACKGROUND The mainstay of diagnosis of relapsing fever (RF) is demonstration of the spirochetes in Giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by microscopy. This study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spirochetemia. METHODS Animal blood samples w...

AIM To determine whether two recently described staining methods (the modified McMullen's and the Helicobacter pylori silver stain HpSS methods) used for the histological identification of H pylori organisms are superior to two established techniques (the modified Giemsa and anti-H pylori antibody immunostain) in terms of availability, reproducibility, rapidity, sensitivity, and cost. METHODS...

A study was carried out in order to compare the detection of Trichomonas gallinae by examination smears throat swab and culture protozoa collected from both pigeon chicken. In present study, five media viz. Modified Diamond’s Media, Nutrient broth, Medium 199, Minimum Essential (MEM) Roswell Park Memorial Institute-1640 (RPMI-1640) were used for T. gallinae. Microscopic wet mount/ Giemsa staine...