Integrating photoactive proteins with synthetic nanomaterials holds great promise in developing optoelectronic devices whereby light, captured by a antenna protein, is converted to modulated electrical response. The protein–nanomaterial interface critical defining properties; successful integration of bionanohybrids requires control over protein attachment site and detailed understanding its im...

Macro biomolecules are of vital importance in regulating the biofunctions organisms, which proteins (including peptides when mentioned below) and nucleic acids (NAs) most important. Therefore, these NAs can be applied as “drugs” to regulate from abnormal normal. Either for NAs, challenging thing is avoid biodegradation or physicochemical degradation before they reach targeted location, then fun...

Transient expression of proteins in plants has become a choice to facilitate recombinant protein production with its fast and easy application. On the other hand, host defensive mechanisms have been reported to reduce the efficiency of transient expression in plants. Hence, this study was designed to evaluate the effect of cap analog and Potato virus A helper component proteinase (PVA HC-Pro) o...

Olomoucine is known as a cyclin-dependent kinase inhibitor. We found that olomoucine blocked insulin's ability to stimulate glucose transport. It did so without affecting the activity of known insulin signaling proteins. To identify the olomoucine-sensitive kinase(s), we prepared analogs that could be immobilized to an affinity resin to isolate binding proteins. One of the generated analogs inh...

Since the cloning of Aequorea victoria green fluorescent protein (GFP) in 1992, a family of known GFP-like proteins has been growing rapidly. Today, it includes more than a hundred proteins with different spectral characteristics cloned from Cnidaria species. For some of these proteins, crystal structures have been solved, showing diversity in chromophore modifications and conformational states...

The formation of protein aggregates is a hallmark neurodegenerative diseases. Observations on patient samples and model systems demonstrated links between aggregate declining mitochondrial functionality, but causalities remain unclear. We used Saccharomyces cerevisiae to analyze how processes regulate the behavior aggregation-prone polyQ derived from human huntingtin. Expression Q97-GFP rapidly...

Transporter associated with antigen processing (TAP)-like (TAPL; ABCB9) is a half-type ABC transporter with sequence similarity to TAP1 and TAP2 that function in the ER membrane. To determine the cellular localization, TAPL and truncated forms of it were tagged with GFP at their carboxyl termini. Intracellular localization of these fusion proteins was compared between transient and stable expre...

The glucagon-like peptide-1 receptor (GLP-1 receptor) mediates important effects on peripheral tissues and the central nervous system. It seems one of the most promising therapeutic targets for treatment of diabetes mellitus type 2. Surprisingly, very little is known about the cellular mechanisms that regulate its function in vivo. One of the approaches to study receptor dynamics, expression, o...