The aim of the present study was to examine the fertilization ability and mitochondrial function of oocytes derived from cows with or without liver damage. Oocytes were collected from the ovaries of cows with damaged livers (DL) and those of cows with healthy livers (HL), subjected to in vitro maturation, and fertilized in vitro. A significantly high abnormal fertilization rate was observed for...

background: cryopreservation and in vitro maturation (ivm) of oocyte is becoming an important technique in infertility treatment and fertility preservation. also it has been proposed to establish a genetic resource bank for endangered or commercially important animal species. objective: the aim of this study was to evaluate viability, maturation and fertilization rate of mouse immature oocytes ...

The requirement of mitogen-activated protein kinase (MAPK) for the initiation of meiotic maturation in porcine oocytes was examined using U0126, an inhibitor of MAPK activator. Immunoblot analysis and kinase assay showed that U0126 inhibited the phosphorylation and activation of MAPK incompletely in porcine oocytes. In this condition, maturation/M-phase promoting factor (MPF) activity was also ...

Background: The effects of cumulus cells and the effectiveness of a PDE3 specific inhibitor (Cilostamide) in comparison with an adenylate cyclase activator (Forskolin) on the maturation rate and developmental competence of mouse germinal vesicle (GV) oocytes were evaluated Materials and Methods: Germinal vesicles (GV) oocytes of PMSG-primed mice were divided into cumulus denuded oocytes (CDOs) ...

To study the intranuclear localization of the Ul-specific snRNP C protein and its assembly into U1 snRNPs, we injected transcripts encoding a myctagged C protein into amphibian oocytes. The distribution of protein translated from the injected RNA was essentially the same in continuous and pulse-label experiments. In both cases the C protein localized within the germinal vesicle in those structu...

Cytoskeletal proteins such as actin and myosin are important constituents of the nucleoplasm. Spectrin is an actin binding protein typically related to plasma membrane; recently, it has been found that it is widespread and forms distinct membrane protein domains in such organelles as the Golgi. In this paper, the large germinal vesicle of amphibian oocytes was chosen as a particularly suitable ...

To study the intranuclear localization of the U1-specific snRNP C protein and its assembly into U1 snRNPs, we injected transcripts encoding a myc-tagged C protein into amphibian oocytes. The distribution of protein translated from the injected RNA was essentially the same in continuous and pulse-label experiments. In both cases the C protein localized within the germinal vesicle in those struct...

in the present study, a total of 595 specimens collected monthly in the northern part of persian gulf between november 2006 and october 2007 were examined by routine macroscopic and histological techniques. monthly changes in the gonadosomatic index showed the spawning season extended within 2 peaks, from april- may and september. the maturity stages are morphologically separated according to t...