Gene therapy is an experimental treatment being investigated to correct defective genes that are responsible for disease development. To treat cancer and genetic illnesses, researchers looking into a variety of gene techniques. Finding proper vector transfer DNA tissues one the most difficult aspects therapy. Some vectors have issues with infecting both quiescent dividing cells, provoking immun...

the immune system of mammalian organisms undergoes alterations that may account for an increased susceptibility to certain infections, autoimmune diseases, or malignancies. well characterized are age related defect in t cell functions and cell mediated immunity. although it is well established that the functional properties of t cells decrease with age, its biochemical and molecular nature is p...

Adeno‑associated virus (AAV) is a common vector utilized in gene therapy. The NIH/3T3 cell line, which is a potential induced pluripotent stem (iPS) cell type, was identified to be a less-permissive cell type to AAV due to its defective endosomal processing. Ultrasound‑targeted microbubble destruction (UTMD) enhanced the gene transduction of AAV in permissive cells. However, there are no data c...

Evidence for a relationship between overexpression of wild-type p53 and telomerase activity remains controversial. We investigated whether p53 gene transduction could cause telomerase inhibition in pancreatic cancer cell lines, focusing on the relation of transduction to growth arrest, cell cycle arrest, and apoptotic cell death. The cells were infected with recombinant adenovirus expressing wi...

Recombinant retroviral vectors are still the most common gene delivery vehicles for gene therapy purposes, especially for construction of genetically modified tumor vaccines (GMTV). However, these vehicles are characterized by relatively low titre and in the case of many tumor cell lines, low transduction efficiency. We constructed bicistronic retroviral vector pseudotypes of amphotropic murine...

UNLABELLED The biological function of the SART-1 gene product is demonstrated and its potential as a target for cancer gene therapy is discussed. MATERIALS AND METHODS The SART-1 gene was transduced by a recombinant adenovirus vector and its expression was promoted by a CMV promoter. RESULTS The transduction efficiency by recombinant adenoviruses in A549 and MCF-7 cells was determined using...

The objective of this research was to understand the expression characteristics and biological functions Medicago truncatula genes under long-day conditions. leaves “R108” tribulus at branch stage (A), bud (B), initial flowering (C), full (D) were sequenced using RNA-Seq technology. genome truncatula, a related species used as reference for sequence comparison. transcriptomes three adjacent per...

The reduction of crop yield caused by soil salinization has become a global problem. Halophytes improve saline alkali soil, and the halophyte transcription factors that regulate salt stress are crucial for improving tolerance. In this study, 1466 were identified transcriptome sequencing analysis Glaux maritima leaves after (0, 600, 800 mM/L NaCl). Their genes distributed across 57 factor famili...

BACKGROUND Hematopoietic stem cells (HSC), in particular mobilized peripheral blood stem cells, represent an attractive target for cell and gene therapy. Efficient gene delivery into these target cells without compromising self-renewal and multi-potency is crucial for the success of gene therapy. We investigated factors involved in the ex vivo transduction of CD34(+) HSCs in order to develop a ...