Lipoteichoic acids (LTAs) of varying chemical composition from five streptococcal species and one lactobacillus species initiated gelation of Limu lus amebocyte lysate. Preincubation with antisera specific for the poly(glycerol phosphate) (PGP) chain or, when appropriate, with antisera, specific for a carbohydrate substituent inhibited gelation initiated by lipoteichoic acid, but did not inhibi...

The thermoreversible gelation of methylcellulose in aqueous solution has been studied by static and dynamic light scattering (DLS), small-angle neutron scattering (SANS), and rheology. At 20 °C, dilute solution light scattering establishes the molecular weight, second virial coefficient, radius of gyration, and hydrodynamic radius of the polymer. Semidilute solutions exhibit two relaxation mode...

There is a medical need for robust, biocompatible hydrogels that can be rapidly crosslinked in situ through the use of gentle and non-toxic triggers, which could be used as a surgical adhesive, a bone-inductive material, or for drug and gene delivery. The complete gelation system described here includes calcium-loaded liposomes, hrFactor XIII. thrombin, and an enzymatic substrate based on a fou...

By William A. Eaton, James Hofrichter, and Philip D. Ross T HE FACTORS which determine the frequency of crises and overall severity in sickle cell disease are still not clear. Although Allison,’ Charache and Conley,2 and others3’4 have considered that the kinetics of sickling may be important clinically, accurate measurements of the rates of hemoglobin S gelation and cell sickling have only bee...

the present work investigated the preparation of biodegradable beads with alginate polymer by ionotropic gelation method to improve the control release properties of the antibiotic rifampicin. ionotropic gelation method was applied to prepare beads using calcium chloride (cacl2) as cationic component and alginate as an anionic component. in this method, adding 0.5% w/v polyvinyl alcohol (pva) t...

We present an approximation scheme to the master kinetic equations for aggregation and gelation with thermal breakup in colloidal systems with variable attraction energy. With the cluster fractal dimension df as the only phenomenological parameter, rich physical behavior is predicted. The viscosity, the gelation time, and the cluster size are predicted in closed form analytically as a function ...

The main aim of the present investigation was to develop in situ gelling mucoadhesive liquid suppositories of ondansetron by using mucoadhesive polymers such as sodium alginate, methyl cellulose and polyvinylpyrrolidone. Polyethylene glycol was used to modify gelation temperature. Mucoadhesive liquid suppositories of ondansetron were prepared by adding mucoadhesive polymers (0.8% w/w) to the fo...

The reaction process and corresponding mechanism of coagulation and gelation of native soy protein isolates (SPIs) induced by microbial transglutaminase (MTGase) were investigated. The protein constituents of SPIs, including a majority of subunits of b-conglycinin and acidic subunits of glycinin, could be polymerized by MTGase to form high weight molecular (WM) biopolymers. Both the coagulation...

The aim of this study was the formulation and examination of a novel thermoresponsive and bioadhesive, in situ gelling drug delivery system, which can be used in the treatment of oesophageal pain and inflammation. A bioadhesive cellulose derivative (Metolose) 60SH) was used as a thermoresponsive material, because Metolose has thermal gelation properties at certain temperature. The thermal gelat...

The Dictyostelium discoideum gelation factor is a two-chain actin-cross-linking protein that, in addition to an N-terminal actin-binding domain, has a rod domain constructed from six tandem repeats of a 100-residue motif that has an immunoglobulin fold. To define the architecture of the rod domain of gelation factor, we have expressed in E. coli a series of constructs corresponding to different...