The zygomycete Phycomyces blakesleeanus has a 30 Mb genome with a 35% content of guanine and cytosine (GC). We determined the GC content in Phycomyces genes and fragments of genes available in public databases, the frequency of nucleotides in each codon position, and the codon usage. We observed a difference of 18% between the GC content of protein-coding and non-coding DNA. This large differen...

Submit Manuscript | http://medcraveonline.com biological entity, such as a chromosome (Figure 1). In a linear biological entity, such as a chromosome; the biological entity is represented on the horizontal axis and the score, such as GC content, is represented on the vertical axis. This result in a line graph representing the GC content across the entire chromosome, which can then be used to id...

Submit Manuscript | http://medcraveonline.com biological entity, such as a chromosome (Figure 1). In a linear biological entity, such as a chromosome; the biological entity is represented on the horizontal axis and the score, such as GC content, is represented on the vertical axis. This result in a line graph representing the GC content across the entire chromosome, which can then be used to id...

Studies throughout the years revealed several characteristics that differentiate alternative exons from constitutive ones. However, some of those characteristics, such as splice signals and other regulatory sequences, are inherently sequence-based. Herein we show that these characteristics are heavily biased by GC content, and may not actually differ between alternative and constitutive exons. ...

Submit Manuscript | http://medcraveonline.com biological entity, such as a chromosome (Figure 1). In a linear biological entity, such as a chromosome; the biological entity is represented on the horizontal axis and the score, such as GC content, is represented on the vertical axis. This result in a line graph representing the GC content across the entire chromosome, which can then be used to id...

Mammalian and avian genomes comprise several classes of chromosomal segments that vary dramatically in GC-content. Especially in chicken, microchromosomes exhibit a higher GC-content and a higher gene density than macrochromosomes. To understand the evolutionary history of the intra-genome GC heterogeneity in amniotes, it is necessary to examine the equivalence of this GC heterogeneity at the n...

High-throughput sequencing is subject to sequence dependent bias, which must be accounted for if researchers are to make precise measurements and draw accurate conclusions from their data. A widely studied source of bias in sequencing is the GC content bias, in which levels of GC content in a genomic region effect the number of reads produced during sequencing. Although some research has been p...

Kent et al. (1) recently investigated genome evolution in the honey bee and proposed “strong links between recombination, biased gene conversion, GC-content evolution, and worker behavior.” However, we feel these connections may be more nuanced than suggested. Kent et al. (1) illustrated dramatically higher guanine and cytosine (GC) content for worker-biased genes than for queen-biased and dron...

Given the dramatic variation in guanine-cytosine (GC) content observed in prokaryotes, from approximately 20% to approximately 75% GC, one wonders if these extreme biases in base pair composition affect the evolution of transcription factor-binding sites (BS). This letter shows that, along the wide range of GC content variation in bacteria, bacterial BS keep a high frequency of AT bases, roughl...