Gardnerella vaginalis, a pleomorphic Gram variable bacteria (1), with a Gram positive cell wall (2). that stains predominantly as Gram negative is associated in bacterial vaginosis (1). We have described a lysozyme-SDS-proteinase K method for the isolation of DNA from 250 ml Casman broth cultures requiring about 60 to 72 hours (3). However, this was not convenient for comparative restriction en...

Florence Bretelle ([email protected]) Florence Fenollar ([email protected]) Karine Baumstarck ([email protected]) Cécile Fortanier ([email protected]) Jean-François Cocallemen ([email protected]) Valérie Serazin ([email protected]) Didier Raoult ([email protected]) Pascal Auquier ([email protected]) Sandrine ...

BACKGROUND Bacterial vaginosis (BV) is one of the leading causes of vaginal complaints among women of childbearing age. The role of Gardnerella vaginalis remains controversial due to its presence in healthy and BV-type vaginal microflora. The phenotypic and genotypic heterogeneity of G. vaginalis suggested the existence of strain variants linked with different health conditions. We sought to an...

A rapid biochemical method for the identification of Gardnerella vaginalis has been developed. The method is based on the fermentation of starch and raffinose and on the hydrolysis of hippurate. With this new procedure, identification was confirmed for 390 of 396 G. vaginalis isolates within 1 h after their inoculation into the three substrates.

OBJECTIVES Bacterial vaginosis (BV) is characterised by a change in the microbial composition of the vagina. The BV-associated organisms outnumber the health-associated Lactobacillus species and form a polymicrobial biofilm on the vaginal epithelium, possibly explaining the difficulties with antibiotic treatment. A better understanding of vaginal biofilm with emphasis on Atopobium vaginae and G...

Gardnerella vaginalis was ingested and killed by neutrophils in the presence of normal human serum. Heat inactivation of the serum inhibited these processes. The opsonisation of some but not all G vaginalis strains was enhanced by immune rabbit serum. Immune serum did not, however, enhance intracellular killing. Blockade of the classical pathway of complement activation had no effect on the ops...

Clinical isolates of Gardnerella vaginalis, Streptococcus agalactiae, Bacteroides spp., and Mobiluncus spp. were screened for resistance to tetracycline and for the presence of the streptococcal tetM determinant. The S. agalactiae and G. vaginalis strains contained DNA sequences homologous to the tetM determinant, while strains of the other two genera did not.

OBJECTIVE To compare the Affirm VPIII Microbial Identification Test for detection and identification of Candida species, Gardnerella vaginalis and Trichomonas vaginalis to clinical and microscopic criteria commonly used to diagnose vaginitis. METHODS Women that were symptomatic for vaginitis/vaginosis and asymptomatic women being seen for routine obstetric or gynecological care were included ...

Pregnant women are prone to vaginal infection. At Primary Health Care Clinics diagnosis of causative organism of vagincll infections is made by clsing historp-taking and assessment of clinicalpicture methods. The accuracy of these methods is investigated in this stctdy by comparing the results obtained by historytaking and assessment of clinical picture with that which is obtained by examining ...