Hepatic carcinoma (HCC) is a lethal disease associated with high morbidity and poor prognosis. Recently years, gene therapies have offered novel modalities to improve the prognosis of HCC patients. MicroRNA-99a (miR-99a) is frequently down-regulated in HCC, where it acts as a tumor suppressor. Therefore, we constructed monomethoxy (polyethylene glycol)-poly(D,L-lactide-co-glycolide)-poly(L-lysi...

In 1962, Elizabeth Peal and Wallace Lambert of McGill University published a monograph entitled “The Relation of Bilingualism to Intelligence.”The research, conducted in Montreal with 10-year-old children, compared the performance of monolinguals t o that of bilingual, French/English-speaking subjects on a variety of standard tests of intelligence. In contrast to previous research on bilinguali...

Pisum sativum (Family: Fabaceae), as known as green pea or garden pea, has long been important in diet due to its content of fiber, protein, starch, trace elements, and many phytochemical substances. It has been shown to possess antibacterial, antidiabetic, antifungal, anti-inflammatory, antihypercholesterolemia, and antioxidant activities and also shown anticancer property. Its nonnutritive bi...

The propeptide form of the lectin from the garden pea (Pisum sativum agglutinin) has been expressed in Escherichia coli by attaching its cDNA to an inducible promoter. By a number of criteria, including the ability to form dimers, hemagglutination titer, Western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteoly...

Aspergillus oryzae glucoamylases encoded by glaA and glaB, and Rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast Saccharomyces cerevisiae GRI-117-UK and laboratory yeast S. cerevisiae MT8-1. Among constructed transformants, GRI-117-UK/pUDGAA, displaying glaA glucoamylase, produced the most ethanol from liquefied starch, although MT8-1/pUDGAR, displaying R. oryzae gl...

Concentration and time course dependence of two structurally related plant flavonoids naringenin and quercetin on the nodABC gene transcriptional activity (as reporter gene nodC-lacZ activity) in Rh. leguminiosarum bv. vicae strain D923 and respective growth, nodulation and nitrogen fixing responses of plant host (garden pea) to inoculation with pre-induced strains were studied. Naringenin was ...

Low-energy conformers of five α-(1,4)and α-(1,6)-linked glucosyl trisaccharides were flexibly docked into the glucoamylase active site using AutoDock 2.2. To ensure that all significant conformational space was searched, the starting trisaccharide conformers for docking were all possible combinations of the corresponding disaccharide low-energy conformers. All docked trisaccharides occupied sub...

Simultaneous extraction and purification of glucoamylase and protease produced concomitantly by Aspergillus awamori Nakazawa MTCC 6652 in a single fermentor using solid-state fermentation (SSF) has been studied. Soaking for 2 h at room temperature (around 30 °C) in 10 % glycerol was found to be most suitable for optimum simultaneous extraction of glucoamylase and protease with the yield of 8645...

The apparent digestibility coefficients (ADC) of unprocessed and processed pea, canola and flax products were determined on diets, in which 30% of a reference diet was replaced by each test ingredient. Celite was used as an indigestible marker for measuring apparent digestibility coefficients indirectly. The trial was conducted in a semi-closed recirculating system using 5 tanks per diet with 4...