ATP-dependent phosphorylation of [14C]galactose by 11 strains of Streptococcus thermophilus indicated that these organisms possessed the Leloir enzyme, galactokinase (galK). Activities were 10 times higher in fully induced, galactose-fermenting (Gal+) strains than in galactose-nonfermenting (Gal-) strains. Lactose-grown, Gal- cells released free galactose into the medium and were unable to util...

The polysaccharide exuded by Acacia decurrens trees contains residues of D-galactose , LArabinose , L-Fucose and Dglucuronic acid. An examination of the OMethyl derivatives of the gum yields 2,3,4,6 tetra –O-methyl –D-galactose, 2,3 –di-O-Methyl –L-arabinose , 2,3,4-triO-methyl-D-galactose, 2,4-di-O-methyl-D-galactose together with 2,3,4 tri-O-methyl-Dglucuronic acid by acid hydrolysis. The deg...

BACKGROUND Duarte galactosemia (DG) is frequently detected in newborn-screening programs. DG patients do not manifest the symptoms of classic galactosemia, but whether they require dietary galactose restriction is controversial. We sought to assess the relationships of selected galactose metabolites (plasma galactose, plasma galactitol, erythrocyte (RBC) galactitol, RBC galactonate, and urine g...

Galactose in serum and galactose-1-phosphate in erythrocytes were measured in six transferase deficient children to determine if these metabolites could be used in detecting transferase deficient galactosemia. In all six children the galactose levels were normal and the galactose-1-phosphate elevated. The galactose level depends on diet and the rate of metabolism to galactose-1-phosphate and, t...

In most organisms, the conversion of -D-galactose to the more metabolically useful glucose 1-phosphate is accomplished by the action of four enzymes that constitute the Leloir pathway (Scheme 1). In the first step of this pathway, -D-galactose is epimerized to -D-galactose by galactose mutarotase. The next step involves the ATP-dependent phosphorylation of -D-galactose by galactokinase to yield...

FUJIMURA, Y., KAWAMURA, M. and NARUSE, H. Simultaneous Quantitative Estimation of Galactose-1-Phosphate and Galactovve in Blood for the Diagnosis of Galactosemia. Tohoku J. exp. Med., 1982, 137 (3), 28°-295 A new microfluorometrical simultaneous assay method of galactose-l-phosphate and galactose in blood discs was devised by use of alkaline phosphatase and fl-galactose dehydrogenase. Our metho...

Galactose oxidase from Fusarium graminearum IMV-1060 adsorbed on, and covalently bound to, silica carriers has been used for analytical determinations of D-galactose and galactose-containing sugars. Using a flowing oxygen electrode of the Clark-type, sensor system for enzymatic analysis of water solutions of galactose-containing carbohydrates was made. Measurements were taken both in the pulse ...

Sørensen M, Munk OL, Mortensen FV, Olsen AK, Bender D, Bass L, Keiding S. Hepatic uptake and metabolism of galactose can be quantified in vivo by 2-[F]fluoro-2-deoxygalactose positron emission tomography. Am J Physiol Gastrointest Liver Physiol 295: G27–G36, 2008. First published May 15, 2008; doi:10.1152/ajpgi.00004.2008.—Metabolism of galactose is a specialized liver function. The purpose of ...

\\hen the diet of chicks contains 15% n-galactose, physical manifestations occur, particularly in female chicks, which include epileptiform seizures and death (1, 2). Galactose and uridine diphosphate galactose accumulate in blood and liver whereas uridine diphosphate glucose levels are greatly reduced (3). Toxicity of dietary galactose in excess has been attributed to n-glucose starvation (4) ...