In this work we have characterized the galA gene product from Pseudomonas putida KT2440, a ring-cleavage dioxygenase that acts specifically on gallate to produce 4-oxalomesaconate. The protein is a trimer composed by three identical subunits of 47.6 kDa (419 amino acids) that uses Fe2+ as the main cofactor. The gallate dioxygenase showed maximum activity at pH 7.0, and the Km and Vmax values fo...

Apples (Malus domestica) are one of the most widely grown and consumed fruits in world that contain abundant phenolic compounds possess remarkable antioxidant potential. The current study characterised from five different varieties Australian apples (Royal Gala, Pink Lady, Red Delicious, Fuji Smitten) using LC-ESI-QTOF-MS/MS quantified through HPLC-PDA. content potential were determined various...

Elsewhere, we reported the safety and efficacy results of a multicenter phase 3 trial of recombinant human alpha -galactosidase A (rh-alpha GalA) replacement in patients with Fabry disease. All 58 patients who were enrolled in the 20-wk phase 3 double-blind, randomized, and placebo-controlled study received subsequently 1 mg/kg of rh-alpha GalA (agalsidase beta, Fabrazyme, Genzyme Corporation) ...

Apples (Malus domestica, Borkh.) which are not stored at low temperature or in a properly controlled atmosphere (CA) may have a high metabolic rate during the postharvest stage resulting in losses in quality. The aim of this study was to evaluate the quality of ‘Royal Gala’ apple fruit stored in accordance with a new method of dynamic controlled atmosphere (DCA).The respiratory quotient (RQ) wa...

For comparative studies we have used the somatic cell hybridization approach to regionally map genes on the mouse X chromosome. Fibroblasts from a mouse with the balanced reciprocal translocation T(XD;16B5)16H were fused with a Chinese hamster cell line (V79/380-6) deficient in activity of the enzyme hypoxanthine phosphoribosyltransferase (HPRT). Interpecific cell hybrids were initially selecte...

We investigated the recruitment and survival of the multimammate mouse, Mastomys natalensis, within irrigated rice and fallow field habitats at different time periods related to rice crop growth stages. Capture-Mark-Recapture data were collected for M. natalensis each month from June 2010 to May 2012, and both recruitment and survival were estimated in relation to land use (irrigated rice or fa...

The generation and selection of novel fire blight resistant apple genotypes would greatly improve the management of this devastating disease, caused by Erwinia amylovora. Such resistant genotypes are currently developed by conventional breeding, but novel breeding technologies including cisgenesis could be an alternative approach. A cisgenic apple line C44.4.146 was regenerated using the cisgen...

The aim in high-resolution connectomics is to reconstruct complete neuronal connectivity in a tissue. Currently, the only technology capable of resolving the smallest neuronal processes is electron microscopy (EM). Thus, a common approach to network reconstruction is to perform (error-prone) automatic segmentation of EM images, followed by manual proofreading by experts to fix errors. We have d...

Microwave assisted extraction (MAE) tech-nique was employed for the extraction of pectin from black mulberry (Morus nigra) pomace in this research. The effects of process variables namely microwave power (300-900 watt), irradiation time (10-30 min) and liquid to solid ratio (15-30 mL/g) on the yield, degree of esterification (DE) and galacturonic acid content (GalA) of pectin were investigated ...

Microwave assisted extraction (MAE) tech-nique was employed for the extraction of pectin from black mulberry (Morus nigra) pomace in this research. The effects of process variables namely microwave power (300-900 watt), irradiation time (10-30 min) and liquid to solid ratio (15-30 mL/g) on the yield, degree of esterification (DE) and galacturonic acid content (GalA) of pectin were investigated ...