Nine different Fusarium species were isolated from Acacia koa seeds, seedpods, seedling stem and root tissues, rhizosphere soil and soil surrounding roots of diseased trees. Fusarium oxysporum, the cause of koa dieback and wilt, was not isolated from seeds or seedpods but was found most commonly on roots and soil near diseased koa seedlings. Fusarium subglutinans was the most common species iso...

Differential display with three time points revealed that thiram altered expression of numerous genes in the biocontrol fungus Fusarium oxysporum CS-20. Of the 101 bands purified from the differential display gel, 86 were successfully cloned, and 64 sequenced. Based on nucleic acid sequences, homology to known products was found using BLASTn for 26 sequences and homology to hypothetical protein...

Fusarium wilts of strawberry, caused by Fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. Fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. Reliable chemical control of strawberry Fusarium wilt disease is not yet available. Moreover, other well-known F. oxysporum have different genetic information fro...

The rhizosphere microbial community in a hydroponics system with multiple parallel mineralization (MPM) can potentially suppress root-borne diseases. This study focused on revealing the biological nature of the suppression against Fusarium wilt disease, which is caused by the fungus Fusarium oxysporum, and describing the factors that may influence the fungal pathogen in the MPM system. We demon...

The impact of 10 Fusarium species in concomitant association with Rotylenchulus reniformis on cotton seedling disease was examined under greenhouse conditions. In experiment 1, fungal treatments consisted of Fusarium chlamydosporum, F. equiseti, F. lateritium, F. moniliforme, F. oxysporum, F. oxysporum f.sp. vasinfectum, F. proliferatum, F. semitectum, F. solani, and F. sporotrichioides; Rhizoc...

The currently available morphological and molecular diagnostic techniques for Fusarium redolens and the three phylogenetic clades of Fusarium oxysporum are problematic. Aligned translation elongation factor 1 alpha (TEF-1 alpha) gene sequences from these species and their close relatives were used to design F. redolens-specific primers, and to identify restriction sites that discriminate among ...

The antifungal compound 2,4-diacetylphloroglucinol-producing bacterium, Pseudomonas fluorescens strain LRB3W1, inhibits the growth of Fusarium oxysporum f. sp. lycopersici, and controls Fusarium wilt of tomato caused by F. oxysporum f. sp. lycopersici. On the other hand, Serratia marcescens strain B2, which produces cell wall-degrading enzyme chitinases, did not inhibit fungal growth and the su...

We used an in silico approach to survey and compare microsatellites in transcript sequences of four sequenced members of genus Fusarium. G + C content of transcripts was found to be positively correlated with the frequency of SSRs. Our analysis revealed that, in all the four transcript sequences studied, the occurrence, relative abundance and density of microsatellites varied and was not influe...

Wilt and crown rot of sweet basil (Ocimum basilicum), caused by Fusarium oxysporum f. sp. basilici, represents a major problem on this crop, which is grown in Italy over a large area (approximately 80 ha) under glasshouse, mainly in the Riviera Ligure (5). First reported in Russia (12), F. oxysporum f. sp. basilici has been observed in Italy (6,22), France (15), the United States (1,2,7,24), an...

Acacia koa seedlings were evaluated from plantings and commercial nurseries for colonization by Fusarium spp., particularly F. oxysporum, the putative cause of koa wilt/dieback disease in Hawaii. We sampled 162 seedlings, 90% of which were from nurseries on the islands of Hawaii, Maui, Kauai, and Oahu. Fusarium oxysporum was detected on about 78% of sampled seedlings. It was most prevalent on r...