The purpose of this study was to determine the shear compliance of human crystalline lenses as a function of age and frequency. Dynamic mechanical analysis was performed on 39 human lenses, ranging in age from 18 to 90 years, within the frequency range of 0.001-30 Hz. The lenses were stored at -70 degrees C before being measured. The influence of freezing on the mechanical properties was determ...

Isolated tomato (Lycopersicon esculentum var. Kc 146) fruit mitochondria could be stored successfully in the frozen state without a cryoprotective agent if the mitochondria were frozen quickly by immersion in liquid nitrogen and later thawed quickly at 30 C. Criteria of freezing damage were rate of respiration, adenosine diphosphate to oxygen ratio, and respiratory control ratio. Marked reducti...

Plants show complex adaptations to freezing that prevent cell damage caused by cellular dehydration. Lipid remodeling of cell membranes during dehydration is one critical mechanism countering loss of membrane integrity and cell death. SENSITIVE TO FREEZING 2 (SFR2), a gene essential for freezing tolerance in Arabidopsis, encodes a galactolipid remodeling enzyme of the outer chloroplast envelope...

The effect of freezing on the properties of a raw ewes'-milk semi-soft cheese (Serpa cheese) was studied using small amplitude oscillatory (SAOS) and texture measurements, colour and chemical parameters. The freezing was introduced at three different stages of the ripening process (28, 35 and 42 days), and the cheeses were maintained frozen for 12 months. Cheeses were submitted to a slow or fas...

Anhydrobiotic animals can survive the loss of both free and bound water from their cells. While in this state they are also resistant to freezing. This physiology adapts anhydrobiotes to harsh environments and it aids their dispersal. Panagrolaimus davidi, a bacterial feeding anhydrobiotic nematode isolated from Ross Island Antarctica, can survive intracellular ice formation when fully hydrated...

Freezing is considered an effective means to preserve fruits without using any chemical preservatives. In this study, the effects of ripeness levels and two freezing temperatures (-25oC -86oC) on frozen pineapples were evaluated. The faster rate (0.4 oC/min) was obtained by -86oC system achieve core temperature -18oC in pineapple ~0.65 h compared that at -25oC with respective 0.09 oC/min time ~...

A quantitative urea-based amyloid b (Ab)-sodium dodecyl sulfate-polyacrylamide gel electrophoresis with Western immunoblot (Ab-SDS-PAGE/immunoblot) reveals highly conserved and disease-specific Ab peptide patterns (Ab 1-37, 1-38, 1-39, 1-40, 1-42) in Alzheimer’sdisease (AD)patientsandnondementedcontrols. For further standardization of this method, we analyzed cerebrospinal fluid (CSF) of eight ...

in order to determine the genetics of barley lines for freezing resistance based on crown survival percentage an experiment was carried out as a split plot design on the basis of randomized complete blocks with two replications. forty barley lines were assigned in subplots and five freezing temperatures (-8, -11, -13, -15 and -17 ˚с) were allocated in main plots. the results showed significant ...

Moss, C. Wayne (North Carolina State University, Raleigh), and M. L. Speck. Release of biologically active peptides from Escherichia coli at subzero temperatures. J. Bacteriol. 91:1105-1111. 1966.-Freezing and storage of Escherichia coli at -20 C in phosphate buffer resulted in loss of cell viability and a pronounced leakage of cellular material which had maximal absorption at 260 mmu. Greater ...

Freeze-induced cell tensions were determined by cell water relations in leaves of broadleaf evergreen species and cell cultures of grapes (Vitis spp.) and apple (Malus domestica). Cell tensions increased in response to cold acclimation in leaves of broadleaf evergreen species during extracellular freezing, indicating a higher resistance to cell volume changes during freezing in cold-hardened le...