The main objective of this study was to isolate high-quality total ribonucleic acid (RNA) from raw fresh semen and frozen-thawed boar semen, using a protocol comprising the conventional TRIzol assay and a membrane-based technique, the PureLink RNA mini kit. Bioanalyzer profile revealed that the sperm RNA size distributions comprised mainly intact RNA ranging from 1500 to 1800 bp, without any de...

The present study was conducted to investigate the effects of butylated hydroxytoluene (BHT) supplementation on diluted, cooled and frozen-thawed ram spermatozoa. After primary evaluation of collected ejaculates, only semen samples with motility of more than 70% and sperm concentration higher than 3×10 sperm/ml were used for cryopreservation. The selected semen samples were then pooled and dilu...

The present study was performed to compare the expression of sperm proteins, i.e. triosephosphate isomerase (TPI) and acrosin binding protein (ACRBP) seminal plasma glutathione peroxidase 5 (GPX5) fibronectin 1 (FN1), in boar semen with good, moderate poor freezability. conducted by determining contents 32 samples 38 semen. ejaculated divided into two portions: first portion centrifuged separat...