نتایج جستجو برای: free fetal dna cffdna

تعداد نتایج: 1076072  

Journal: :Clinical chemistry 2012
Angela N Barrett Thomas C R McDonnell K C Allen Chan Lyn S Chitty

BACKGROUND Cell-free fetal DNA (cffDNA) constitutes approximately 10% of the cell-free DNA in maternal plasma and is a suitable source of fetal genetic material for noninvasive prenatal diagnosis (NIPD). The objective of this study was to determine the feasibility of using digital PCR for NIPD in pregnancies at risk of sickle cell anemia. METHODS Minor-groove binder (MGB) TaqMan probes were d...

Journal: :Human reproduction 2005
Phillip D Pan Inga Peter Geralyn M Lambert-Messerlian Jacob A Canick Diana W Bianchi Kirby L Johnson

BACKGROUND Increased second-trimester levels of maternal serum HCG in IVF conceptions lead to an increased false-positive rate in Down syndrome screening. Increased levels of cell-free fetal DNA (cffDNA) in maternal plasma have been correlated with increased HCG levels. Our aim was to determine whether cffDNA levels are elevated in IVF pregnancies compared with natural pregnancies. METHODS Si...

Journal: :Journal of obstetrics and gynaecology of India 2015
Chanchal Singh Anita Kaul

Recent advances in genomic sequencing and bioinformatics have led to the development of noninvasive detection methods with detection rates approaching those obtained with amniocentesis and chorionic villus sampling (CVS) [1, 2]. Recently, a novel prenatal testing method has become available. This method, known as noninvasive prenatal testing (NIPT), is a molecular approach for assessing fetal a...

Journal: :Clinical genetics 2011
M Hill K Finning P Martin J Hogg C Meaney G Norbury G Daniels L S Chitty

The effectiveness and clinical utility of non-invasive prenatal diagnosis (NIPD) for fetal sex determination using cell-free fetal DNA (cffDNA) was assessed by undertaking a prospective national audit of UK testing. NIPD was performed using real-time polymerase chain reaction analysis of the DYS14 or SRY gene in cffDNA extracted from maternal plasma. All cases referred for fetal sex determinati...

2017
Carlo Vermeulen Geert Geeven Elzo de Wit Marjon J.A.M. Verstegen Rumo P.M. Jansen Melissa van Kranenburg Ewart de Bruijn Sara L. Pulit Evelien Kruisselbrink Zahra Shahsavari Davood Omrani Fatemeh Zeinali Hossein Najmabadi Theodora Katsila Christina Vrettou George P. Patrinos Joanne Traeger-Synodinos Erik Splinter Jeffrey M. Beekman Sima Kheradmand Kia Gerard J. te Meerman Hans Kristian Ploos van Amstel Wouter de Laat

During pregnancy, cell-free DNA (cfDNA) in maternal blood encompasses a small percentage of cell-free fetal DNA (cffDNA), an easily accessible source for determination of fetal disease status in risk families through non-invasive procedures. In case of monogenic heritable disease, background maternal cfDNA prohibits direct observation of the maternally inherited allele. Non-invasive prenatal di...

Journal: :Human reproduction 2012
Megan Allyse Lauren C Sayres Jaime S King Mary E Norton Mildred K Cho

The recent release of new, non-invasive prenatal tests for fetal aneuploidy using cell-free fetal DNA (cffDNA) has been hailed as a revolution in prenatal testing and has triggered significant commercial interest in the field. Ongoing research portends the arrival of a wide range of cffDNA tests. However, it is not yet clear how these tests will be integrated into well-established prenatal test...

2011
Maryam Zargari Mohammad Reza Sadeghi Mohammad Hassan Shahhosseiny Koroush Kamali Kyomars Saliminejad Ali Esmaeilzadeh Hamid Reza Khorram Khorshid

In previous years, identification of fetal cells in maternal blood circulation has caused a new revolution in non-invasive method of prenatal diagnosis. Low number of fetal cells in maternal blood and long-term survival after pregnancy limited the use of fetal cells in diagnostic and clinical applications. With the discovery of cell-free fetal DNA (cffDNA) in plasma of pregnant women, access to...

2017
Bin Zhang Bei-Yi Lu Bin Yu Fang-Xiu Zheng Qin Zhou Ying-Ping Chen Xiao-Qing Zhang

Objective To explore the feasibility of high-throughput massively parallel genomic DNA sequencing technology for the noninvasive prenatal detection of fetal sex chromosome aneuploidies (SCAs). Methods The study enrolled pregnant women who were prepared to undergo noninvasive prenatal testing (NIPT) in the second trimester. Cell-free fetal DNA (cffDNA) was extracted from the mother's peripheral ...

Journal: :International journal of women's health and reproduction sciences 2021

Objectives: This study aimed to evaluate the association between cell-free fetal DNA (cffDNA) levels in first and second trimesters adverse pregnancy outcomes. Materials Methods: was a retrospective cohort including all women who had cffDNA measurement as primary or secondary screening test for chromosomal abnormalities laboratory Iran. Results: Totally, 481 out of 634 pregnant (75.9%) did not ...

Journal: :international journal of fertility and sterility 0
leili moezzi zeinab keshavarz reza ranjbaran farzaneh aboualizadeh abbas behzad-behbahani masooma abdullahi

objective: maternal-fetal rhd antigen incompatibility causes approximately 50% of clinically significant alloimmunization cases. the routine use of prophylactic anti-d immunoglobulin has dramatically reduced hemolytic disease of the fetus and newborn. recently, fetal rhd genotyping in rhd negative pregnant women has been suggested for appropriate use of anti-d immunoglobulin antenatal prophylax...

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