Convergent syntheses of polyglycerol hyperbranched polymers containing fluorescent labels (fluorescein or perylene diimide (PDI)) at their core are presented. The hyperbranched polyglycerol (HPG) precursors were synthesized using a one step polymerization reaction wherein the initiator leaves a single reactive group for dye functionalization. For further site isolation, allylated HPG was synthe...

Cryogenic fluid flows including liquid nitrogen and superfluid helium are a rich environment for novel scientific discovery. Flows can be measured optically and dynamically when faithful tracer particles are dispersed in the liquid. We present a reliable technique for dispersing commercially available fluorescent nanoparticles into cryogenic fluids using ultrasound. Five types of fluorescent na...

The overproduction of hydrogen peroxide is implicated in the development of numerous diseases and there is currently great interest in developing contrast agents that can image hydrogen peroxide in vivo. In this report, we demonstrate that nanoparticles formulated from peroxalate esters and fluorescent dyes can image hydrogen peroxide in vivo with high specificity and sensitivity. The peroxalat...

this study discusses the effect of complexes of chitosan grafted polyethylenimine(ch-pei) with plasmid dna on viability of mesenchymal stem cells(mscs) derived from human marrow. ch-pei/pdna nanoparticles were synthesized through the complex coacervation method using pires plasmid containing green fluorescent protein (gfp) gene. to confirm the complexation, samples were run through an agarose g...

Oils containing both fluorescent semiconductor and magnetic oxide nanoparticles are used to produce oil in water emulsions. This technique produces oil droplets with homogeneous fluorescence and high magnetic nanoparticle concentrations. The optical properties of the oil droplets are studied as a function of the droplet sizes for various concentrations of fluorescent and magnetic nanoparticles....

Based on chelation-enhanced fluorescence, a new fluorescent coumarin derivative probe 3(1-(7-hydroxy-4-methylcoumarin)ethylidene)hydrazinecarbodithioate for Hg(2+), Ag(+) and Ag nanoparticles is reported. Fluorescent probe acts as a rapid and highly selective "off-on" fluorescent probe and fluorescence enhancement by factors 5 to 12 times was observed upon selective complexation with Hg(2+), Ag...

Magnetic nanoparticles and fluorescent quantum dots (QDs) can make many effective applications in biomedical system. Here, we demonstrated one way of synthetic method and its surface modification to use for biomedical applications. Fe3O4 nanoparticles are well known as magnetic materials and its magnetic property can be used in magnetic resonance imaging (MRI), cell detection. QDs as a fluoresc...

A novel rolling circle amplification (RCA) immunoassay based on DNA enriching magnetic nanoparticles and assembled fluorescent DNA nanotags, magnetic nanoparticles-RCA immunoassay, is developed as a versatile fluorescence assay platform for highly sensitive proteins detection.

The first successful approach to synthesizing ultrabright fluorescent mesoporous silica nanoparticles is reported. Fluorescent dye is physically entrapped inside nanochannels of a silica matrix created during templated sol-gel self-assembly. The problem of dye leakage from open channels is solved by incorporation of hydrophobic groups in the silica matrix. This makes the approach compatible wit...

Because of their multifunctionality and unique magnetic properties, superparamagnetic iron oxide nanoparticles (SPIONs) have been recognized as very promising materials for various biomedical applications. The main difficulty with the use of SPIONs as multimodal bioimaging agents is their lack of fluorescence. Since cells can act as extremely efficient filters for the elution of surface-bound f...