The cDNA clone, CanMADS1, was isolated from young flower buds of the hot pepper (Capsicum annuum L.) by screening a cDNA library using the OsMADS1 rice MADS-box gene as a probe. We used a yeast two-hybrid screening method to investigate interaction partners of the protein product of CanMADS1. A MADS-box gene, CanMADS6, was isolated from young flower buds using the region containing the K domain...

Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA is a frequent occurrence, its molecular mechanism remains unknown. A transcript-derived fragment (TDF72), which was obtained by cDNA amplified fragment length polymorphism (cDNA-AFLP), was up-regulated in the aborted buds and exhibited 89% sequence homology with the AtγVPE gene. In...

Dioxygenase, Flavonoid Biosynthesis, Flavonols, Genetic Control, Petunia hybrida Soluble enzyme preparations from flower buds of Petunia hybrida catalyzed the conversion of dihydroflavonols to flavonols. Dihydrokaempferol and dihydroquercetin were readily converted to the respective flavonols, whereas dihydromyricetin was a poor substrate. The reaction required 2-oxoglutarate, ascorbate and Fe2...

Protein extracted from boll weevil larvae causes abscission of cotton flower buds and debladed petioles. Abscission of buds is preceded by a rapid and persistent flaring of bracts; cell proliferation is induced in the abscission zone of debladed petioles. A possible mode of transmission of the agent from larva to bud tissue is discussed.

Dormancy is an adaptive mechanism that allows temperate deciduous plants to survive unfavorable winter conditions. In the present work, we investigated the possible function of abscisic acid (ABA) on the endodormancy process in pear. The ABA content increased during pear flower bud endodormancy establishment and decreased towards endodormancy release. In total, 39 putative genes related to ABA ...

Grafting is a well-established practice to facilitate asexual propagation in horticultural and agricultural crops. It has become a method for studying molecular aspects of root-to-shoot and/or shoot-to-root signaling events. The objective of this study was to investigate differences in gene expression between the organs of the scion and rootstock of a homograft (Arabidopsis thaliana). MapMan an...

cDNA library constructed from male flower buds of Populus deltoides Bartr. ex Marsh. According to an analysis of genomic DNA structure and putative protein structure, and a phylogenetic study, PdPI is an ortholog of the Arabidopsis PI gene. Relative-quantitative real-time polymerase chain reaction analysis showed that PdPI has a broader expression pattern than PI in Arabidopsis. PdPI was strong...

The influence of late-season boron (B) application on the bud B concentration and fruit set was studied in sweet cherry (Prunus avium L.). The experiment was carried out on 5-year-old ‘Summit’ and ‘Hedelfinger’ trees on rootstock Gisela 5 in Fruit Growing centre Gačnik. Trees were sprayed with B (1% Bortrack) or water (control). Differences in B concentration were measured between cultivars, th...

Eucalyptus nitens (Deane & Maiden) Maiden takes at least five years to initiate flower buds from seed and is an infrequent and light flowerer. Because this behavior constitutes a major impediment to breeding programs, we examined the mechanisms controlling floral induction in E. nitens, with the long-term aim of reducing generation time and increasing seed yield. Application of paclobutrazol re...

The influence of scoring during the flower induction phase was investigated. Pear trees, Pyrus communis L. cv. Doyenne du Comice, Forelle and Rosemarie were used in the study, conducted in the Western Cape, South Africa. The yield of ‘Doyenne du Comice’ increased by 38% in scored trees compared to the control. The increase in yield was due to 50% more fruits on the scored trees than on the cont...