An autosomal dominant missense mutation in αB-crystallin (αB-R120G) causes cataracts and desmin-related myopathy, but the underlying mechanisms are unknown. Here, we report the development of an αB-R120G crystallin knock-in mouse model of these disorders. Knock-in αB-R120G mice were generated and analyzed with slit lamp imaging, gel permeation chromatography, immunofluorescence, immunoprecipita...

THE choroidal coat of the eye consists of a dense continuous network of vessels and yet it is a striking feature of choroidal disease that the lesions often remain remarkably circumscribed. It is the purpose of this paper to review the problem of selective localization of pathological changes within the choroid in relation to its vascular anatomy, with particular reference to certain findings (...

Light source temperature measurement has been an important aspect in many industrial applications. The aim of this study is to determine the temperature of different sources like flames, incandescent lamps. The sensor installation is difficult in these sources. Imaging these sources to correlate with the temperature is attempted. A digital still camera is used to capture the source images. The ...

PURPOSE To determine the function of the lens fiber cell-specific cytoskeletal protein, filensin, in lens biology. METHODS Targeted genomic deletion was used to delete exon 1 and the transcriptional start site of the filensin gene. Resultant chimeric animals were bred to homozygosity for the mutant allele. These animals were outbred to mice bearing the wild-type CP49 alleles to eliminate the ...

LAMP-1 (lysosome-associated membrane protein), a major glycoprotein present in the lysosomal membrane, constitutes up to 50% of total membrane proteins. LAMP-1, expressed at the plasma membrane, is reported to be the major molecule expressing the sialyl-Lewis X antigen. Two forms of LAMP-1 exist; the full-length LAMP-1 [LAMP-1 (+Tail)] has a highly glycosylated lumenal domain, a membrane-spanni...

background : some characteristics of lighting sources such as color properties and ultraviolet emissions have important roles on visual and non-visual health effects of lighting. this study aimed to investigate the light emissions of some compact fluorescent lamps (cfls) and incandescent lamps commercially available to the iranian consumers. methods: sixty lamps included 48 single envelope cfls...

Loop-mediated isothermal amplification (LAMP) combined with enzyme-linked immunosorbent assay (LAMP-ELISA) and with lateral flow dipstick (LAMP-LFD) are rapid, sensitive and specific methods for the visual detection of clinical pathogens. In this study, LAMP-ELISA and LAMP-LFD were developed for the visual detection of canine parvovirus (CPV). For LAMP, a set of four primers (biotin-labeled for...