Cerf, M. E., K. Williams, X. I. Nkomo, C. J. Muller, D. F. Du Toit, J. Louw, and S. A. Wolfe-Coote. Islet cell response in the neonatal rat after exposure to a high-fat diet during pregnancy. Am J Physiol Regul Integr Comp Physiol 288: R1122–R1128, 2005. First published February 10, 2005; doi:10.1152/ajpregu.00335.2004.—Although pancreatic -cells are capable of adapting their mass in response t...

background: this study was conducted to evaluate fibroblast co-culture and activin a on in vitro maturation and fertilization of mouse preantral follicles. methods: the ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-mem as based medium for 12 days. a total number of 456 follicles were cultured in four conditions: (i) base mediu...

introduction: mineral trioxide aggregate is being widely used for root end filling, pulp capping, perforation repair, and other endodontic procedures. recently, a material similar to proroot mineral trioxide aggregate (pmta) was developed in iran named root mineral trioxide aggregate (rmta) with the claim of having the exact result of original mta. the purpose of this study was to compare the a...

dendritic cells (dcs) induce pathogen-specific t cell responses. we comprehensively studied the effects of addition of maturation stimulus, fibroblasts (fibroblast conditioned medium), pha activated t cells (t cell conditioned medium), and mixture of fibroblast & pha activated t cells (fcm-tccm) conditioned media on maturation of dcs. monocytes were cultured with gm-csf and il-4 for five da...

cytotoxicity of depleted uranium, as a byproduct of military has been came to spotlight in recent decades. du is known as a chemical rather than radioactive hazard and efforts to illustrating its mechanism is undergo, but the precise complete molecular mechanisms are still unclear. recent studies showed that uranium induces biological changes in many different target tissues, such as the kidney...

in order to adapt lasota strain of newcastle disease virus (ndv) on chick embryo fibroblast (cef) cells, 0.1 ml from lasota vaccine produced in razi vaccine and serum research institute inoculated to the cef cells and was passaged five times in the cef grown in eagle’s minimum essential medium (mem). first to third passages were blind but in fourth passage cytopathic effect of virus was observe...

fibroblasts could be considered as connective tissue cells that are morphologically heterogeneous with diverse functions depending on their location and activity. these cells play critical role in health and disease such as cancer and wound by production of collagen, fibronectin, cytokines and growth factors. absence of insulin and other growth factors in serum deprivation condition and similar...

background and aims. since intracanal medicaments can affect the cell viability in periapical tissues, the aim of this study was to evaluate the effect of calcium hydroxide and propolis on pulp fibroblasts. materials and methods. two healthy third molars were used as a source to obtain fibroblasts. the fibroblasts were cultured and subjected to 1 mg/ml of propolis and calcium hydroxide. this ex...