conclusions a fusion tag from the pet32 expression plasmid permits the recovery of the recombinant fusion fgf-2 from e. coli, without affecting its biological activity. objectives the objective of this study was to overexpress and purify the high-level active human bfgf in escherichia coli (e. coli). materials and methods this experimental study was conducted in the islamic republic of iran. af...

objective(s): umbilical cord blood is a good source of the mesenchymal stem cells that can be banked, expanded and used in regenerative medicine.  the objective of this study was to test whether amniotic membrane extract, as a rich source of growth factors such as basic-fibroblast growth factor, can promote the proliferation potential of the umbilical cord mesenchymal stem cells. materials and ...

In the rat spinal cord, we found substantial co-existence of fibroblast growth factor-2, fibroblast growth factor receptor (type-1 or flg) immunoreactivity and reduced nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase activity (a histochemical marker for neuronal nitric oxide synthase) in preganglionic autonomic cell groups of intermediate layers VI, VII and X. Anti-fibroblast grow...

Stromal cell-derived FGF-7 binds and activates only the resident FGFR2IIIb in epithelial cells while FGF-1 and FGF-2 exhibit a broader interaction with multiple isoforms of FGFR. Here we report the structure of FGF-7 that has been solved to 3.1 A resolution by molecular replacement with the structure of a dual function chimera of FGF-7 and FGF-1 (FGF-7/1) which was resolved to 2.3 A. Comparison...

Abbreviations: UV-R: UV Radiation; SCF: Stem Cell Factors; FGF2: Fibroblast Growth Factor; PAH: Phenylalanine Hydroxylase; TH1: Tyrosinase Hydroxylase 1; TRP-2: DOPA-Chrome Tautomerase; TRP-1: DHICA Oxidase; DHI: 5,6-Dihydroxyindole; DHICA: 5;6-Dihydroxyindole-2-Carboxylic Acid; Indole-5;6-Quinone I-Q; I-QCA: Indole-5,6-Quinone Carboxylic Acid; MSH: MelanocyteStimulating Hormone; TYR: Tyrosinas...

background: basic fibroblast growth factor (bfgf) is a glycoprotein with stimulating ability of angiogenesis. in addition, bfgf plays an important role in wound healing process in some tissues for example gastrointestinal tract, but its ability for discrimination of various stages of wound severity in these diseases was not reported. therefore, we aimed to determine the bfgf levels in gastric u...