We have investigated the influence of blood flow on thrombin generation, fibrin formation, and fibrin deposition on procoagulant and nonprocoagulant surfaces. Nonanticoagulated human blood was drawn for 5 minutes directly from an antecubital vein over stimulated endothelial cells expressing tissue factor and over human type III collagen fibrils, positioned in parallel-plate perfusion chambers. ...

• In contracted clots and thrombi, erythrocytes are compressed to close-packed polyhedral structures with platelets and fibrin on the surface. • Polyhedrocytes form an impermeable seal to stem bleeding and help prevent vascular obstruction but confer resistance to fibrinolysis. Contraction of blood clots is necessary for hemostasis and wound healing and to restore flowpast obstructive thrombi, ...

Tissue factor (TF) is expressed on nonvascular cells and cells within the vessel wall and circulates in blood associated with microparticles. Although blood-borne TF accumulates into the developing thrombus during thrombus formation, the contribution of blood-borne TF and vessel wall TF to thrombin generation in vivo following vessel injury is unknown. To determine the source and role of blood-...

T relationship among platelet survival, vessel injury, and thrombosis is of interest because of the role that platelets play in the development of atherosclerosis and its thromboembolic complications. Blood platelets do not adhere to normal endothelium. However, when a blood vessel is injured, platelets adhere to the injury site and, if blood flow is disturbed, thrombi can form on the injured a...

BACKGROUND The advent of fibrin-binding molecular magnetic resonance (MR) contrast agents and advances in coronary MRI techniques offers the potential for direct imaging of coronary thrombosis. We tested the feasibility of this approach using a gadolinium (Gd)-based fibrin-binding contrast agent, EP-2104R (EPIX Medical Inc), in a swine model of coronary thrombus and in-stent thrombosis. METHO...

Factor XIII (FXIII) stabilizes thrombi against fibrinolysis by cross-linking α2-antiplasmin (α2AP) to fibrin. Cellular FXIII (FXIII-A) is abundant in platelets, but the extracellular functions of this pool are unclear because it is not released by classical secretion mechanisms. We examined the function of platelet FXIII-A using Chandler model thrombi formed from FXIII-depleted plasma. Platelet...

BACKGROUND AND PURPOSE The aim of this study is to analyze the histological composition of acute arterial thrombi and their MRI signals. METHODS Two different types of experimental thrombi, erythrocyte- and fibrin-rich thrombus, were created and injected into an experimentally formed stenotic common carotid artery in swine. MRI of the in vivo and in vitro samples was obtained immediately afte...

IN 19461 and subsequently, Duguid2-4 presented evidence derived from human material that thrombosis might cause, not merely complicate, atherosclerosis. This was an independent revival of Rokitansky's original Auflagerung (incrustation) hypothesis.' Duguid considered erythrocytes a source of lipid in atherosclerosis. In 1936, Clark and associates5 had suggested that atherosclerosis arose by dep...

BACKGROUND Activated factor XIII (FXIIIa) mediates fibrinolytic resistance and is a hallmark of newly formed thrombi. In vivo imaging of FXIIIa activity could further elucidate the role of this molecule in thrombosis and other biological processes and aid in the clinical detection of acute thrombi. METHODS AND RESULTS An FXIIIa-sensitive near-infrared fluorescence imaging agent (A15) was engi...

IN 19461 and subsequently, Duguid2-4 presented evidence derived from human material that thrombosis might cause, not merely complicate, atherosclerosis. This was an independent revival of Rokitansky's original Auflagerung (incrustation) hypothesis.' Duguid considered erythrocytes a source of lipid in atherosclerosis. In 1936, Clark and associates5 had suggested that atherosclerosis arose by dep...