This study was designated to describe clinical and biological features of patients with a suspected diagnosis of dengue fever/dengue hemorrhagic fever during an outbreak in Central Vietnam. One hundred and twenty-five consecutive patients hospitalized at Khanh Hoa and Binh Thuan Provincial hospitals between November 2001 and January 2002 with a diagnosis of suspected dengue infection were inclu...

1006 Emerging Infectious Diseases • Vol. 8, No. 9, September 2002 capture enzyme-linked immunosorbent assay) and reverse transcriptasepolymerase chain reaction (RT-PCR) tests for flaviviruses, alphaviruses, and Bunyamwera serogroup bunyaviruses were also negative. RT-PCR for Crimean-Congo hemorrhagic fever virus (C-CHFV) was positive. Tests for anti-C-CHFV–specific IgM antibody by indirect immu...

a 1972 serosurvey suggested widespread distribution of chikungunya infection , and numerous outbreaks have reemerged since 2001 (3). Malaysia reported its fi rst outbreak between De-cember1998 and February 1999 and a reemergence in an isolated northwestern coastal town in 2006 (4). In Singapore, although dengue fever has been endemic since the 1960s, the fi rst chikungunya case was not reported...

In December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiologic agent of disease 2019 (COVID-19), emerged in Wuhan, China. Several variants concern (VOCs) have been identified so far. Recently, B.1.1.529 (Omicron) variant SARS-CoV-2 spread rapidly worldwide. We describe first case Omicron genetic lineage BA.1 our region. The patient is a physician who traveled to J...

PURPOSE To report the first case of Chikungunya encephalitis acquired in the perinatal period during the current outbreak in Brazil. METHODS Case report. RESULTS A male neonate with 3500 g developed macular erythematous rash, hypoactivity and fever progressing to generalized seizures. His mother had experienced a disseminated rash and fever before delivery. EEG showed diffuse slowing and cr...

Classical swine fever (CSF) is a contagious and devastating viral disease, causing serious losses in the pig industry worldwide. In the control programmes of CSF, rapid detection and identification of the causative agent is a crucial step. Various PCR based techniques like nested RT-PCR, SYBRGreen based RealTime PCR and TaqMan based Real-time PCR were used for detection of CSFV nucleic acid in ...

In this article, the development of a new TaqMan-based one-step real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for detection and quantification of Crimean-Congo hemorrhagic fever virus (CCHFV) RNA is described. Selected oligos targeting the highly conserved S region of CCHFV were designed by using our oligo design and analysis software, Oligoware 1.0. None of the prime...

Aim: The urokinase-type plasminogen activator (uPA) system consists of a protease, receptor (urokinase-type receptor, uPAR), and inhibitors that can be expressed on various cell types. Previous literature shows the amount soluble (suPAR) secreted from affected cells is higher in Crimean-Congo hemorrhagic fever (CCHF) patients than healthy controls. Thus, we aimed to investigate diagnostic value...

Background: Coronavirus Disease 2019 (COVID-19) is an infectious disease caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2). SARS-CoV-2 a new type of corona virus that has never been previously identified in humans. There are at least two types coronavirus known to cause diseases can severe symptoms, such as Middle East (MERS) and (SARS). WHO recommends molecular testing for all patient...