OBJECTIVE To assess whether a significant correlation exists between the echographic measurement of biventricular outer diameter and the pretransfusional serum concentration of fetal hemoglobin and whether that echographic measurement can be used as a noninvasive marker of fetal anemia. METHODS A prospective cross-sectional study was carried out comprising 65 cordocenteses performed in 36 ane...

Standard hematologic methods were used.2 In vitro sickling tests were carried out using the method of 1)aiand and Castle.’ Fetal hemoglobin was estimated by the alkali denaturation technic of Singer, Chernoff and Singer.’ Filter-paper electrophoresis of hemoglobin was performed in a horizontal tank using barbitone buffer pH 8.6 and ionic strength about 0.02. The technic is described in detail e...

ETICULOCYTES of several species have been shown to accumulate amino acids, and incorporate them into hemoglobin.12 While the ratio of the concentration of certain amino acids in mature human red cells to the plasma concentrations of the same amino acids has been studied,1 there has been no study of this ratio in human reticulocytes. Because of the presence of large amounts of fetal hemoglobin i...

The in uitm biosynthesis of acetylated human fetal hemoglobin (Hb FI) was investigated in umbilical cord blood from two normal newborns and in peripheral blood from five adults with different hematologic disorders accompanied by elevated levels of fetal hemoglobin. Hemoglobin biosynthesis was measured by [8HJleucine incorporation into hemoglobin components which were separated by chromatograph...

BACKGROUND The development of therapies for sickle cell disease has received special attention, particularly those that reduce the polymerization of hemoglobin S. Hydroxyurea is a commonly used medication because it has the ability to raise levels of fetal hemoglobin, decrease the frequency of vaso-occlusive episodes and thus improve the clinical course of sickle cell disease patients. OBJECT...