1. Peripheral blood mononuclear cells from eight healthy volunteers were cultured, with or without concanavalin A (Con A), in a medium containing (ml/l) 100 normal autologous serum, 100 experimental autologous serum or 100 heterologous (fetal calf) serum. 2. The control and experimental autologous sera were obtained from the volunteers, before and after 15 d supplementation with 15 g fish oil (...

The solid agar overlay procedure used for viral plaquing was adapted to the study of Pseudomonas aeruginosa plaques in HeLa monolayers. After adsorption of the organism to the HeLa monolayers, an overlay consisting of 10% newborn calf serum (NBCS), 90% Eagles basal medium (EBM), and 1.5% agar containing neutral red was added. Plaques developed after 24 hr of incubation at 37 C which were indist...

Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoligonucleotides are protected from exonuclease degradation in sera and cell media by s...

many investigators have used xenogeneic, especially murine stromal cells and fetal calf serum to maintain and expand human stem cells. the proliferation and expansion of human hematopoietic stem cells in ex vivo culture were examined with the goal of generating a suitable protocol for expanding hematopoietic stem cells for patient transplantation. using primary fetal liver cells, we established...

Ehrenwörtliche Versicherung Diese Dissertation wurde selbständig, ohne unerlaubte Hilfe erarbeitet. I Abbreviations µM micromolar APS ammoniumperoxodisulfate BSA bovine serum albumin cDNA copy DNA cpm counts per minute C-terminal carboxyterminal dATP desoxyadenosintriphosphate dCTP desoxycytosintriphosphate DEPC diethylpyrocarbonate DNA desoxyribonucleicacid dNTP desoxynucleosidtriphosphate DTT...

in this study the programmed cell death (apoptosis) of human neutrophilic granulocytes was investigated in the presence and absence of fetal calf serum (fcs), cycloheximide and actinomycin-d. the results show that when fcs is omitted from cultures, apart from a decrease in viability, the percentage of apoptotic cells and dna fragmentation increases. apoptosis is accelerated in serum withdrawal ...

cells as described previously.2} Fctal calf serum (Walkerville, MD) was delipidated by tl'ic method described by Chani and Knowles.3) The cells were routine]y maintained in Eagle7$ minimum essential medium with 6va) de]jpidated fetal calf serum (medium A) and ineubated at 37"C gncler a 5V{i C02 atmosphcre at 1OO% rclative humidity, The cetls were seeded in the mediurn A at 1.8 x 104 cellsi'cm2....

Two human hepatoma cell lines, Hep G2 and Hep 3B, were screened for vitamin D3-25-hydroxylase enzyme activity by incubation with radioactive vitamin D3. A compound co-chromatographing with 25-OH-D3 was synthesized in both cell lines but its rate of synthesis was tenfold greater in Hep 3B than in Hep G2 cells. The identity of the compound was confirmed by comparing its chromatographic properties...