OBJECTIVE To prepare the PrP specific monoclonal antibodies (mAbs) that can be used for the detection of mammalian prions and study of pathogenesis of prion diseases. METHODS Several BALB/c mice were immunized with recombinant hamster prion protein (HaPrP). Three hybridoma cell lines designated as B7, B9, and B10, secreting monoclonal antibodies against HaPrP, were established by hybridoma te...

Ribosomes from Mycobacterium tuberculosis (Mtb) possess species-specific ribosomal RNA (rRNA) expansion segments and ribosomal proteins (rProtein). Here, we present the near-atomic structures of the Mtb 50S ribosomal subunit and the complete Mtb 70S ribosome, solved by cryo-electron microscopy. Upon joining of the large and small ribosomal subunits, a 100-nt long expansion segment of the Mtb 23...

Interleukin (IL) 11 is a multifunctional cytokine derived from bone marrow stromal cells. To understand the mechanisms by which IL-11 exerts its pleiotropic actions, we have analyzed IL-11-mediated signal transduction pathways in IL-11-dependent B9-TY1, which is a subclone of an IL-6-dependent B-cell hybridoma, B9. IL-11 stimulation of B9-TY1 cells resulted in tyrosine phosphorylation of a 97/9...

The human endometrial adenocarcinoma HEC-1-B cell line was transfected with an expression vector harboring the human basic fibroblast growth factor (bFGF) cDNA under the control of the human beta-actin gene promoter. Stable transfectants were obtained in which a constitutive, limited overexpression of M(r) 24,000, 22,000, and 18,000 bFGF isoforms was observed. When transfectants were screened f...

INTRODUCTION Oxidative stress is a key factor in the development of the principal comorbidities of obesity. Methylenetetrahydrofolate reductase enzyme (MTHFR) participates in the metabolism of folate with the action of vitamins B6 and B12. The gene of MTHFR may present a single nucleotide polymorphism (SNP) at position 677 (C677T), which can promote homocysteinemia associated to the production ...

Marrubium eriocephalum (Lamiaceae) is described as a new record for the Flora of Turkey (B9 Van). A detailed morphological description, photographs, distribution map, and pollen and nutlet morphology of this new record are given.

A variable rate fertilizer system was developed for control of fertilizer amount by optical sensor measured NDVI of crop. The paper analyzes the input and output conditions of control system, and designed hardware, algorithm and control of fertilizer, mainly software flow and a feedback control way. In the paper, the variable-rate control system consisted of 6 optical sensors mounted on a boom ...

Precision agriculture is the developing trend of modern agriculture, and the rational utilization of fertilizer is one of the key technologies in the precision agriculture, which needs to fertilize variably according to the crop needs and soil fertility conditions. Thus a fertilizer automated proportioning equipment is developed to Proportion the three fertilizers: Nitrogen fertilizer, Phosphor...

Just after transplanting, the vegetable has difficulty in nutrients uptake. To explore the effect of different fertilization on spring cabbage (Brassica oleracea L. var. capitata) production and fertilizer use efficiencies, this experiment consisting of six treatments was implemented and divided into three groups: 1) no fertilizer (NF) and vegetable planting fertilizer (VPF); 2) conventional fe...