The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whereas volumetric productivities differ largely. In the fed-batch cultivations final rTfH concentra...

Introduction Nowadays, single-use bioreactors are widely accepted in pharmaceutical industry. This is based on shorter batch to batch times, reduced cleaning effort and a significantly lower risk of cross contaminations [1,2]. One large field of the application of single-use bioreactors is the seed train cultivation of mammalian cells [1]. The focus is further extended to perform state of the a...

This review is a synopsis of different bioprocess engineering approaches adopted for the production of marine enzymes. Three major modes of operation: batch, fed-batch and continuous have been used for production of enzymes (such as protease, chitinase, agarase, peroxidase) mainly from marine bacteria and fungi on a laboratory bioreactor and pilot plant scales. Submerged, immobilized and solid-...

BACKGROUND In comparison with phototrophic growth, heterotrophic conditions can significantly increase growth rates, final cell number and cell mass in microalgae cultures. Neochloris oleoabundans is a microalga of biotechnological interest that accumulates lipids under phototrophic and nitrogen-limited conditions. Heterotrophic flask culture experiments were conducted to identify carbon source...

White wine fermentations are typically performed in an entirely batchwise manner, with yeast nutrients only added at the beginning of fermentation. This leads to slow (2+ weeks) fermentation cycle times, large capital expenditures required increase winery processing capacity. Prior attempts speed via increasing temperature have resulted unpalatable wine, and continuous is uneconomical impractic...

Considering the hybrid nature in fed-batch culture of glycerol biconversion to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae, we propose a state-based switching dynamical system to describe the fermentation process. To maximize the concentration of 1,3-PD at the terminal time, an optimal switching control model subject to our proposed switching system and constraints of continuous state ine...

Fed-batch culture is often used in industry, and dissolved oxygen (DO) concentration control is important in fermentation process control. DO control is often applied by using feedback (FB) control strategy. But, feedforward-feedback (FFFB) control has the advantage in dealing with the time-varying characteristics resulting from cell growth during the fermentation process. Mathematical modeling...

Paja Brava is a native South American grass with a high carbohydrate content. In the current work, the potential of using this feedstock for ethanol production using a simultaneous saccharification and fermentation (SSF) process with the xylose-fermenting yeast Pichia stipitis (Scheffersomyces stipitis) CBS6054 was investigated. The straw material was subjected to SO2 catalyzed steam pretreatme...

level: intermediAte Most biopharmaceutical production platforms are based on fed-batch cell culture protocols, which can support high volumetric productivity while maintaining low operational complexity (1). The industry is interested in developing or refining high-titer cell culture processes to meet increasing market demands and reduce manufacturing costs (2). Although advancements in cell en...