objective: utility of pcr-rflp and species-specific pcr as novel and fast methods for identification and discrimination of causative agents of relapsing fever, borrelia persica and b. microtii in infected blood were investigated. materials and methods: genomic dna of b.persica and b.microtii species were extracted from the highly infected blood samples. two fragments of glpq and 16srdna genes ...

A retrospective analysis of real-time PCR (RT-PCR) results for 151 biopsy samples obtained from 132 patients with proven invasive fungal diseases was performed. PCR-based techniques proved to be fast and sensitive and enabled definitive diagnosis in all cases studied, with detection of a total of 28 fungal species.

American foulbrood (AFB) is the most serious brood disease of the honey bee. Traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. The aim of this study is the detection of Paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and PCR. Therefore 54 samp...

PURPOSE Polymerase chain reaction (PCR) assay, introduced as a fast and sensitive diagnostic method, is useful in detecting Mycobacterium tuberculosis. The purpose of this study was to evaluate the usefulness of in-house PCR assay in the detection of Mycobacterium tuberculosis by comparing PCR results with conventional diagnostic techniques and Cobas Amplicor M. tuberculosis kit. MATERIALS AN...

ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of pathogen are important for detection presence parasite in this food. This study aimed optimize polymerase chain reaction (PCR)-based T. cruzi DNA pulp. Several dilutions DTU TcI trypomastigote fo...

Nucleic acid amplification is enormously useful to the biotechnology and clinical diagnostic communities; however, to date point-of-use PCR has been hindered by thermal cycling architectures and protocols that do not allow for near-instantaneous results. In this work we demonstrate PCR amplification of synthetic SARS respiratory pathogenic targets and bacterial genomic DNA in less than three mi...

The routine detection of large and medium copy number variants (CNVs) is well established. Hemizygotic deletions or duplications in the large Duchenne muscular dystrophy DMD gene responsible for Duchenne and Becker muscular dystrophies are routinely identified using multiple ligation probe amplification and array-based comparative genomic hybridization. These methods only map deleted or duplica...

Background: Nocardia is a Gram-positive and partially acid-fast bacterium. The species are widely distributed in the environment cause severe human infections. Nocardiosis not easily identifiable due to lack of pathognomonic clinical signs. Objectives: present study was designed develop evaluate simple quick method based on loop-mediated isothermal amplification (LAMP) assay for detecting spp i...

background : in this study, we used both its1 and its2 for molecular identification of fasciola species. methods : the region between 18s and 28s of ribosomal dna was used in pcr-rflp method for molecular identification of fasciola species. ninety trematodes of fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from khorasan, east azerbaijan, a...