Two series of highly fluorinated 3-substituted hydrotris(indazolyl)borate (Fn-Tp(4Bo,3R); R = Ph, n = 12; R = CF3, n = 21) complexes of calcium were synthesized by salt metathesis reactions. CaI2 reacts with 2 and 1 equiv. of Tl(F12-Tp(4Bo,3Ph)) in THF to form the homo- and heteroleptic complexes [Ca(F12-Tp((4Bo,3Ph)*))2] (1) and [(F12-Tp(4Bo,3Ph))CaI(THF)] (2), respectively. 1 has C2h symmetry...

By using polyethylene glycol 1540, BW5147 AKR T lymphoma cells were fused with splenocytes from A/J mice treated so as to induce suppressor T cells specific for azobenzenearsonate (ABA). Of 576 microwells originally seeded, 132 demonstrated growing cell clones, 4 of which produced an ABA-binding supernatant factor. When tested in vivo for suppression of delayed-type hypersensitivity to ABA, two...

Geometric and spectroscopic parameters are determined using explicitly correlated coupled cluster ab initio calculations (CCSD(T)-F12) for five monosubstituted isotopologues of acetone containing 1...

A realistic double many-body expansion potential energy surface (PES) is developed for the 2A″ state of carbon–nitrogen–oxygen (CNO) system based on MRCI-F12/cc-pVQZ-F12 ab initio energies. The new PES reproduces fitted points with chemical accuracy (root mean square deviation up to 0.043 eV) and explicitly incorporates long range terms that can accurately describe electrostatic dispersion inte...

AIM To find an in vitro system for the measurement of the androgenic effects of different extracts of Hibiscus macranthus (Malvaceae) and Basella alba (Basellaceae). METHODS The production of testosterone from testes slices incubated in two media, either Krebs-Henseleit buffer containing 0.5% Bovine serum albumin (BSA) or Dubecco's Modified Eagle's medium-F12 Ham nutrient mixture (DME/Ham F12...

OBJECTIVE To investigate the possible association between factor XII (F12) gene variant and the presence of coronary artery disease (CAD) in Chilean subjects. METHODS A total of 112 unrelated patients with a diagnosis of CAD confirmed by angiography (33-74 years old) and 107 healthy controls (30-68 years old) were included in this study. PCR-RFLP was used to evaluate the 46C --> T polymorphis...

We have isolated and characterized three anti-recombinant human erythropoietin (rHuEPO) monoclonal antibodies (MoAbs) that recognize nonoverlapping epitopes on rHuEPO. Anti-EPO MoAb D11 neutralizes rHuEPO activity whereas MoAbs F12 and 9G8A do not. This suggests that D11 may bind to the rHuEPO active site. MoAbs F12 and D11 recognize conformation dependent epitopes whereas 9G8A does not. Immuno...

A system for organ culture of rat bladder in defined medium, without serum, is described. Transitional epithelial morphology of normal bladder was well maintained in Waymouth's Medium MB 752/1, while Ham's Medium F12 caused marked epithelial hyperplasia. Hyperplastic epithelial states induced by in vivo administration of the carcinogen, N-methyl-N-nitrosourea, were well maintained in vitro duri...

The equation-of-motion coupled-cluster method for excited states with the singles-and-doubles model (CCSD) has been implemented for ansatz 2 of the explicitly correlated CCSD(R12) and CCSD(F12) methods as part of the program package Dalton. In this model, an orthonormal complementary auxiliary basis set is used for the resolution-of-identity approximation in order to calculate the three-electro...

Glioblastoma stem cells are able to reform original glioblastoma and express the neural stem cell marker CD133 and Nestin. They can self-renew and proliferate in tumor sphere medium containing EGF, bFGF and LIF that is known to be permissive for stem cell proliferation. In this study, we found that neurosphere-like colonies appeared after the human primary glioblastoma cells had been switched i...