An extraction method is described for quantitatively determining Mg, K, Na, 2n, and Mn in plant tissues. The tissue is extracted with 2% trichloroacetic acid and the elements are determined on the filtrate by atomic absorption spectrophotometry. Results compare favorably with those determined after wet ashing with nitric and perchloric acids. In addition, trichloroacetic acid extracts the same ...

The present study was intended to determine the concentrations of phenolic compounds in surface waters of Godavari canal by molecular spectrophotometry. Samples were collected from fifteen sampling stations using grab sampling method for a period of four months (from November to February) at 10 day intervals. Total phenols in water samples were determined using molecular spectrophotometry after...

Laser micro-dissection permits isolation groups of cells from heterogeneous tissue sections under direct microscopic visualization. This technology not only allows isolation of homogenous subpopulation of cells but also allows immunophenotypically similar cells for RNA/ DNA or protein extraction for downstream applications like western blot, mass spectrophotometry, real time quantitative polyme...

We describe a method for the 7-min determination of theophylline in less than 100 muL of serum. The procedure requires no centrifugation or solvent evaporation after extraction. Butylation is done on the gas-chromatographic column by injecting the serum extract followed by a butylating mixture which contains 1-iodobutane as the alkylating agent. The method is precise, accurate, and free of inte...

Propolis was used as medicine, cosmetic and beverage additives since past decades. The most important active compounds in the propolis are phenolic and flavonoids, which are in the resin category. The purpose of this study was to measure the total amount of flavonoids and quercetin in propolis of northern Iran and compare obtain data with different extraction methods. The soxhlet, maceration an...

BACKGROUND Urinary extracellular vesicles (UEVs) represent an ideal platform for biomarker discovery. They carry different types of RNA species, and reported profile discrepancies related to the presence/absence of 18s and 28s rRNA remain controversial. Moreover, sufficient urinary RNA yields and respective quality RNA profiles are still to be fully established. METHODS UEVs were enriched by ...

There has been a remarkable increase in the interest shown to trace metal contents in various foodstuffs, since various heavy metals have been shown to be invariably present in environment and in the foodstuffs with the development of analytical methods and techniques. Lead and cadmium have been recognized as important environmental pollutants with toxic effects on human at relatively low level...