background and objectives: clostridium perfringens, a gram-positive obligate anaerobic bacterium, is able to form resistant spores which are widely distributed in the environment. c. perfringens is subdivided into five types a to e based on its four major alpha, beta, epsilon and iota toxins. the aim of the present study was cloning and expression of c. perfringens type d vaccine strain epsilon...

classical lbp such as complexity and high dimensions of feature vectors that make it necessary to apply dimension reduction processes. in this paper, we introduce an improved lbp algorithm to solve these problems that utilizes fast pca algorithm for reduction of vector dimensions of extracted features. in other words, proffer method (fast pca+lbp) is an improved lbp algorithm that is extracted ...

Background: Cholera toxin B subunit (CTB) has been extensively considered as an immunogenic and adjuvant protein, but its yield of expression is not satisfactory in many studies. The aim of this study was to compare the expression of native and mutant recombinant CTB (rCTB) in pQE vector. Methods: ctxB fragment from Vibrio cholerae O1 ATCC14035 containing the substitution of mutant ctxB for ami...

the interaction between immune cells and endothelial lining of blood vessels is vital in many processes such as inflammatory and immune responses as well as cancer cell metastasis. the expression level of vcam-1 is regulated by many factors including the promoter activity that is possibly affected by the single nucleotide polymorphisms (snps) present in the promoter. there are previous reports ...

a:4:{s:10:"Background";s:897:"Human luteinizing hormone (hLH) belongs to glycoprotein hormones which is composed of two non-covalently linked subunit, α and β. The α-subunit is similar in all glycoprotein hormones, whereas the β-subunit is conferring the hormonal specificity. This hormone has important roles in the growth and maturity of sexual organs and secondary sexual characteristics and st...

The pLE2SCX vector was developed for the stable expression of exogenous genes in the protozoan parasite Leishmania. The pLE2SCX construct contains three independent selection markers: herpes simplex virus thymidine kinase (HSV-TK), cytosine deaminase (CD) and streptothericin acetyltransferase gene (sat) in multiple cloning site, flanking by 5’ and 3’ untranslated regions of the previously clone...

the ple2scx vector was developed for the stable expression of exogenous genes in the protozoan parasite leishmania. the ple2scx construct contains three independent selection markers: herpes simplex virus thymidine kinase (hsv-tk), cytosine deaminase (cd) and streptothericin acetyltransferase gene (sat) in multiple cloning site, flanking by 5’ and 3’ untranslated regions of the previously clone...

Background: The micro-ribonucleic acids (miRNAs) are noncoding RNA molecules that are conserved developmentally and include usually 18-25 nucleotides. MiRNA regulates gene expression through mRNA degradation or inhibiting of its translation. These biomolecules contribute in cellular physiologic and pathologic processes and most of them may act as oncogenes or tumor inhibitors. Identification of...

expressions of recombinant proteins for different applications are important objectives in molecular biotechnology; however, expression of some recombinant proteins is difficult. several methods have been designed for expression of these proteins. the aim of this study was to construct a vector containing mtb32c fragment of mycobacterium tuberculosis (m.tuberculosis) as a fusion partner in orde...