abstract         background and objective: hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. the aim of this study was to evaluate the effect of inserting protein a signal peptide sequence of pezz18 plasmid on expression and secretion of the recombinant hirudin in e.coli.  ...

results cloning and subcloning of the flic gene were confirmed by colony pcr, restriction enzymes digestion and dna sequencing of the recombinant plasmids pprime-flic and pvax-flic. the expression of flagellin protein in eukaryotic cells was approved by immunofluorescence assay (ifa), western blotting analysis and the reverse transcriptase polymerase chain reaction (rt-pcr) method. conclusions t...

background: recent researches have demonstrated that human t-cell immunoglobulin mucin 1 (tim-1) glycoprotein plays important roles in regulation of autoimmune and allergic diseases, as well as in tumor immunity and response to viral infections. therefore,targeting tim-1 could be a potential therapeutic approach against such diseases.objectives: in this study, we aimed to express tim-1 protein ...

background: the purpose of this study was to clone, express, and purify a novel multidomain fusion protein of micobacterium tuberculosis (mtb) in a prokaryotic system. methods: an hspx/esxs gene construct was synthesized and ligated into a pgh plasmid, e. coli top10 cells were transformed, and the vector was purified. the vector containing the construct and pet-21b (+) plasmid were digested wit...

Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...

background: plants are among promising and suitable platform systems for production of recombinant biopharmaceutical proteins due to several features such as safety, no need for fermentation, inexpensive investment, and fast and easy scale-up. human insulin is one of the most widely used medicines in the world. up to now different expression systems including escherichia coli, yeast and cho hav...