The polyamines putrescine, spermidine (SPD), and spermine are a family of low-molecular-weight organic cations essential for cell growth and differentiation and other aspects of signal transduction. Hypoxic pulmonary vascular remodeling is accompanied by depressed lung polyamine synthesis and markedly augmented polyamine uptake. Cell types in which hypoxia induces polyamine transport in intact ...

Abstract Bioactive compounds extracted from plant are of great value for those enterprises interested in the use natural products; tissue culture techniques guarantee a reliable and constant biomass production. Hop ( Humulus lupulus L.), with its wealth bioactive compounds, may represent an invaluable resource. The present study focused on characterization vitro -derived leaves roots two hop ty...

An epithelial-mesenchymal cell transformation (EMT) occurs during the development of endocardial cushions in the atrioventricular (AV) canal of the heart. This is a complex developmental process regulated by multiple extracellular signals and signal transduction pathways. It was recently shown that the transcription factor Slug is expressed in the AV canal and is required for initial steps of E...

Subunit composition of subsynaptic transmitter receptors is controlled presynaptically in the developing neuromuscular junction. To investigate presynaptic regulation of NMDA receptor subunit composition in the CNS, we co-cultured different types of hippocampal explants with dissociated target neurons. Postsynaptic NMDA receptors were studied using whole-cell patch-clamp recordings. After 1 wee...

explants of leaves, petals, anthers, styles and ovaries of c. speciosus, c. cancellatus, c. gilanicus and c. almehensis were cultured on ls media with three different combinations of naa and bap in the dark. callus and neoformant organs (leaf and corm-like structure and ordinary and contractileroots) were fomd on some of these explants. corm explants from these species were also cultured on ms ...

in the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-d) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (camellia sinenesis l.). embryonic axes and cotyledons explants were dissected from the seeds. explants were cultured on murashige and skoog (ms) medium containing 0, 1 and 5 mm 2, 4-d alone for embryonic axes and 0, 1 and 5 mm 2, 4-d along with 0...

Genetic improvement programs for conifer forest species face the challenge of propagating elite individuals with superior characteristics in present landscape climate change; problem is focused on fact that when these have shown desirable traits, they changed phase and therefore lost ability to be propagated by traditional methods. Based our previous works Pinus spp. regeneration adult trees th...

ÐWe have established a new test concept for in vitro pharmacological trials. Our model employs tissue explants to test compounds for toxicity which arises with the metabolic interactions among di€erent cell types. Microsurgical preparation of tissue explants avoids the destruction of the organ-speci®c tissue architecture. Explants were mounted in tissue carriers to improve nutrition and handlin...

The present work describes an efficient micropropagation and callus induction protocol of Limbarda crithmoïdes L. (Asteraceae), a halophyte species with medicinal horticultural interests. objective was to identify the culture media that support best organogenesis callogenic expressions four types aerial explants; nodal internodal segments leaf portions, by varying mineral composition twenty add...