In cultured mammary explants from both virgin and midpregnant mice which contained a low and a high initial level of putrescine, respectively, addition of an ornithine decarboxylase inhibitor, DL-a-hydrazino-Saminovaleric acid at the onset of culture prevented an early, “osmolarity-sensitive” rise in the putrescine level that occurred prior to induction of DNA synthesis by insulin. In the pres...

embryogenic callus initiation from anther and whole flower explants in four grapevine cultivars including flame seedless, shahroodi, perlette and red sultanina cultured on piv and harst nutrient media was studied. results showed that whole flower explants of ‘flame seedless’ cultured on piv medium had the most potential for callus production. later callus were transferred onto ges1ca medium for...

in order to optimize the micropropagation of qare-qat (vaccinium arctostaphylus j.j.sm), samples were collected from two regions of iran (asalem and kelardasht). anderson (an) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg l-1) and different levels of ph (4.5, 5 and 5.5) were compared. the in vitro shoots were transferred on an media supplemented with zeatin (1 and 2...

Tissue culture is an attractive alternative for plant propagation. Micropropagation is a technique to ensure a constant and uniform source of ornamental plants. Matthiola incana is an important ornamental species mainly cultivate by seed. Matthiola incana seeds were germinated on solid MS medium without plant growth regulators. Shoot proliferation and root formation are possible using kinetin (...

The present study was the first report on somatic embryogenesis of Tunisian chickpea (Cicer arietinum L.) particularly supposed to be recalcitrant and difficult manipulate in vitro. An efficient protocol has been developed for inducing indirect derived from immature zygotic embryo axis, young leaflet hypocotylar explants. Callogenesis achieved full-strength Murashige Skoog's (1962) (MS) basal m...

the first successful and an efficient regeneration system on in vitro direct plant regeneration of agastache foeniculum was reported. the experiment were done in factorial design with three replications and explants were cultured in ms media fortified with different concentrations of bap and tdz (0, 2.2, 4.4, 8.8 μm) in combination with 3 levels of iaa (0, 1.1 and 2.2 μm). anova analysis showed...

Pelargonium quercetorum Agnew grows naturally in the Hakkari province of Türkiye. Although P. has potential use as a medicine and ornamental plant, it is especially used medicinal plant for cure various diseases by local people. In vitro tissue culture methods are favorable propagation, conservation, breeding plants. We aimed this study to achieve regeneration from different explant types. Seed...

for evaluation of citric acid and citrate effects on bovine epididymal protoplasmic droplets, fifty bovine testes were collected in the october 2007 till june 2008 from urmia slaughterhouse and transported to the laboratory in a cool container filled with 5 °c ice pack. caudal epididymis was incised and sperm cells were put into petri dishes containing hams f10 media with 10% fetal calf serum (...

The present study was conducted to induce callus from different in vitro grown seedling explants of shoot, root and leaf segments in order to develop shoot buds and rooting from callus. The sterilized seeds were cultured on Murashige and Skoog medium, and then the explants were cultured from seedling and transferred to an MS medium supplemented with different concentrations of BAP, kinetin, NAA...

Microbial production of citric acid was performed by Yarrowia lipolytica NBRC 1658 and a domestic strain in the media containing glucose or fructose as substrates. The study was carried out in a batch system. The non-competitive substrate inhibition model was proposed for growth of the yeasts in both substrate media. Higher dry mass and lower Ks values were obtained in glucose media when compar...